Calpis Co., Ltd.

Masanori Takase

Hiroaki Murakami

Disclosed is a method for detecting guaiacol-producing bacteria in a specimen comprising culturing the specimen or a dilution thereof on a plate of a solid medium for acidophilic bacteria comprising a compound represented by the following formula, wherein R is &#x2014;H, &#x2014;OH, &#x2014;C(O)H, &#x2014;C(O)CH, &#x2014;COOH, C-Calkyl or C-Calkenyl, wherein alkyl and alkenyl may optionally be substituted by &#x2014;OH, &#x2014;C(O)H or &#x2014;COOH); and detecting a colony formed on the solid medium. According to preferred embodiments, the solid medium for acidophilic bacteria comprises 50 ppm or more of vanillic acid and the plate culture is carried out at 20&#xb0; C. to 55&#xb0; C. The present invention also provides a solid medium for acidophilic bacteria and a kit for detection of guaiacol-producing bacteria in a specimen. According to the present invention, guaiacol-producing bacteria present in a specimen such as fruit juice raw materials can be detected rapidly in a simple manner.

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Kanagawa, Japan

Masanori Takase