Toyo Boseki Kabushiki Kaisha

Takuma Co., Ltd.

Masanori Oka

Atsushi Sogabe

Takahide Kishimoto

Yoshihisa Kawamura

Seiji Takeshima

Yoshiaki Nishiya

Masao Kitabayashi

Shigenori Emi

Tadayuki Imanaka

Kazumi Yamamoto

Shizuo Hattori

Shigeaki Nishii

Noriaki Hirayama

Toshiro Kikuchi

Kazuhiro Matsui

Toshihiro Kuroita

Kenji Inagaki

Takashi Hatta

Hideyuki Komatsubara

Takuya Ishibashi

Hiroki Fujihira

Shizuo Kataoka

Yoshikazu Ishida

Hiroshi Nishise

Hirotsugu Mishima

Tadanobu Matsumura

The disclosure relates to (i) a gene isolated fromsp.JCM2471, the gene encoding a new glycerol kinase and (ii) a method for preparing the glycerol kinase by gene recombination technique, A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant vector, and a method for producing the glycerol kinase, including culturing the transformant to produce glycerol kinase, and collecting the resulting glycerol kinase.

Glycerol kinase, which has high resistance against preservative

PQQGDH having an improved substrate specificity or having an improved specific activity in an assay system using ferricyanide ion as a mediator is provided. Modified PQQGDH having the enhanced substrate specificity by introducing an amino acid mutation in a particular region of PQQGDH, and a method of enhancing the specific activity compared with a wild type in the assay system using the ferricyanide ion as the mediator by deleting, substituting, or adding one or more amino acids in an amino acid sequence of the wild type pyrroloquinoline quinone dependent glucose dehydrogenase.

Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase excellent in substrate specificity

The present invention relates to modified pyrroloquinoline quinone dependent glucose dehydrogenase (PQQGDH) having lower activity with respect to disaccharides and/or greater stability than wild-type PQQGDH.

Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability

The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived fromspecies, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof

A protein having been modified by addition, deletion, insertion or substitution of at least one amino acid in an amino acid sequence constituting a protein having a sarcosine oxidase activity and still having the sarcosine oxidase activity, characterized by having an improved stability in the state of a liquid compared with the unmodified one and/or having a lowered action on L-proline compared with the unmodified one. A sarcosine oxidase having at least one of the following characteristics, i.e., an action on L-proline being 0.7% or less based on sarcosine and a Km value to L-proline being 150 mM or more, when measured at 37&#xb0; C. and pH 8.0; a process for producing sarcosine oxidase having an excellent substrate specificity which comprises culturing a microorganism capable of producing sarcosine oxidase and collecting the sarcosine oxidase from the culture medium; and a reagent for measuring creatinine which contains the sarcosine oxidase.

Modified sarcosine oxidase, process for producing the same and reagent composition using the same

A compound of formula (1) and an immunoassay method for quantitative determination of dioxins in a sample using as a standard the compound of the following formula (1): wherein R, R, Rand Rmay be the same or different and represent chlorine or hydrogen, n is an integer from 1 to 10, and Z represents an amino acid residue or peptide.

Standard compound for immunoassay for dioxin and method of immunoassay for dioxin

The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteine.

Determination method of biological component and reagent kit used therefor

A DNA fragment that contains a gene encoding creatinine amidohydrolase is provided. This invention also provides a recombinant vector containing the said DNA fragment, a transformant containing the said vector, and a method for producing creatinine amidohydrolase by the use of the said transformant.

DNA fragment containing a gene encoding creatinine amidohydrolase

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Tsuruga, Japan

Masanori Oka