The Green Cross Corporation

Masanori Nagai

Hirofumi Arimura

Tadakazu Suyama

Koji Murakami

Haruhide Kawabe

Toshizumi Tanabe

Ryuji Hiramatsu

Shusei Uno

Hiroshi Nakakubo

Yasuo Amatsuji

Masaaki Hirose

Teruo Kaneda

Takeshi Yamauchi

Tsutomu Kitagawa

A CSF-1 gene promoter region is disclosed. This nucleotide base sequence is useful as a promoter for gene expression in animal cells.

CSF-1 gene promoter

A human prourokinase mutant in which the entire or a partial epidermal growth factor domain of human prourokinase is deleted or a partial epidermal growth factor domain of human prourokinase is replaced by one or more different amino acid residues, said mutant having a longer blood half-life than naturally occurring human prourokinase while retaining prourokinase enzymatic activity. In this human prourokinase mutant the region selected from the group consisting of: (a) from asparagine (10) to cysteine (42); (b) from asparagine (10) to aspartic acid (45); and (c) from asparagine (10) to threonine (49) is missing.

Human prourokinase mutants

Double-stranded RNA synthesized using native human DNA as template is found to be an excellent interferon inducer with low toxicity in spite of homologous native to host cell. It is produced by reacting ATP, GTP, CTP and UTP with one another in the presence of a native human DNA as template by the catalytic action of an active RNA polymerase to form RNA and subjecting the resulting RNA to annealing by heating it at a temperature of 70.degree. to 100.degree. C. and gradually cooling to room temperature or below to form double-stranded regions between their molecules.

Interferon inducer and method of preparing same

Growing community of inventors

Kyoto, Japan

Masanori Nagai