Dojindo Laboratories

Daiichi Pure Chemicals Co., Ltd.

Dojindo Laboratories Co., Ltd.

Masanobu Shiga

Kazumi Sasamoto

Munetaka Ishiyama

Masayuki Nozawa

Makoto Mizoguchi

Susumu Ito

Eiji Watanabe

Yoko Miyazono

Masafumi Iwamoto

Hiroshi Takeuchi

Kazuhiro Takesako

Tomoya Tanaka

A method for measuring concentration of dehydrogenase and substrate thereof in the presence of living cells, wherein an electron mediator that is hard to be reduced by living cells, for example, a compound represented by Formula (I), an electron mediator represented by the Formula (I) and a measurement reagent for dehydrogenase and substrate thereof including the electron mediator are disclosed.A-L-M&#x2003;&#x2003;(I), wherein A represents a structural part containing at least one anionic group, M represents a structural part for mediating electron transfer and L represents a linker part for linking the A to the M.

Measurement method for dehydrogenase and substrate concentration thereof, electron mediator, and measurement reagent for dehydrogenase including said electron mediator and for substrate thereof

Water-soluble tetrazolium salt compounds

Diagnostic marker

An object of the present invention is to inhibit the influence of the reducing substances on the oxidative coloring reaction in biological components. A constitution of the present invention is that a compound having the free radical of the general formula (1) ##STR1## is previously added to the biological sample and then an incubation is carried out. An effect of the present invention is that the influence of the reducing substances on the oxidation reaction can be inhibited and, even when no reducing substance is present, there is no effect on the oxidation reaction.

Method of inhibiting the activity of the reducing substances in

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Kumamoto, Japan

Masanobu Shiga