Hitachi Chemical Company, Ltd.

Kanebo, Limited

Masanobu Munekata

Kenji Tajima

Kazuaki Nishimura

Yasuharu Satou

Tokuo Matsushima

Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA. Solution: The new microorganism is capable of producing a polyhydroxyalkanoate comprising a 16S rRNA gene whose polynucleotide sequence shows 99% or more homology to a polynucleotide sequence represented by SEQ ID No: 1, having an optimum temperature of an activity temperature range for the growth and PHA production of the microorganism of at least 45&#xb0; C. and being capable of growing at a pH range from 6 to 10.

Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same

A precementum- and/or cementum-derived chemotactic factor (CCTF) of a tooth of a Mammalian is characterized by a molecular weight measured by SDS-PAGE of 67000&plusmn;1000. A process for purifying a precementum- and/or cementum-derived gingival fibroblast chemotactic factor (CCTF) of a Mammalian tooth includes eluting a protein mixture from precementum and/or cementum of a Mammalian tooth and purifying the precementum- and/or cementum-derived chemotactic factor (CCTF) from the eluted protein mixture by molecular weight fractionation, ion-exchange adsorption chromatography and hydroxyapatite adsorption chromatography. In the process for purifying the gingival fibroblast chemotactic factor (CCTF) the Mammalian is preferably a bovine.

CELL CHEMOTACTIC FACTOR (CCTF) ORIGINATING IN MAMMALIAN TOOTH PRICEMENT OR CEMENT, METHOD FOR PURIFYING THE SAME, AND NOVEL CONNECTIVE TISSUE ADHESION PROMOTERS CONTAINING THE SAME AS THE ACTIVE INGREDIENT

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Sapporo, Japan

Masanobu Munekata