Takara Bio Inc.

Mie University

Masanari Kitagawa

Ikunoshin Kato

Junichi Mineno

Katsuyuki Dodo

Hiroshi Shiku

Sachiko Okamoto

Risa Sumioka

Tamaki Akashika

Tatsuji Enoki

Hiroyuki Mukai

Hikaru Takakura

Hideto Chono

Naoyuki Awazu

Yasuhiro Kawano

Toshihiro Shodai

Shuohao Huang

Takahisa Tsukihara

Mayumi Shimomura

Koichi Inoue

A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.

Method for quantifying adeno-associated virus

Provided is a method for producing non-enveloped viral particles, comprising a step for obtaining a fraction containing non-enveloped viral particles by removing precipitates which are produced in a step for adding a substance which reduces the solubility of proteins under acidic conditions and/or a substance which precipitates under acidic conditions to a neutral or basic sample containing non-enveloped viral particles, and acidifying the sample after the addition of the substance.

Method for producing non-enveloped viral particles

The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.

Expression cassette

Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.

Method for expression of specific gene

A polypeptide having an endonuclease activity derived from a psychrophilic microorganismsp. strain Ac10, which exhibits high activity at low temperatures, can remove any nucleic acid present in a protein solution and can reduce the viscosity of a protein extract; and a nucleic acid encoding the polypeptide.

Microorganism-derived psychrophilic endonuclease

Growing community of inventors

Yasu, Japan

Masanari Kitagawa