Amano Enzyme Inc.

Ensuiko Sugar Refining Co., Ltd.

Bio Research Corporation of Yokohama

Masamichi Okada

Shotaro Yamaguchi

Miho Nagaya

Yukiko Hoshi

Masayuki Hojo

Akio Horii

Naoki Matsumoto

Hitoshi Amano

Yoshiaki Kurono

Akiko Sugita

Hiroyuki Hashimoto

Koki Fujita

Kozo Hara

Shigeharu Mori

Akiko Matsunaga

Atsuki Toumoto

Kazutaka Tsuruhami

Kanzo Sakata

Taichi Usui

Miho Nagoya

Shigeru Yamamoto

The present invention has a purpose of providing a β-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a β-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.

Beta-galactosidase enzymes

The present invention has a purpose of providing a novel β-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a β-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.

The present invention has a purpose of providing a novel &#x3b2;-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a &#x3b2;-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.

A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics: (1) effect: oxidizing a saccharide to produce a saccharic acid; (2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and, (3) [Km value of glucose]/[Km value of maltose]&#x2264;1.

Saccharide oxidase, and production method for same and use of same

Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

Stabilized transglutaminase and process for production thereof

Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of: culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

A novel &#x3b2;-amylase having excellent practical applicability is found, and an object is to provide a practical use of the &#x3b2;-amylase. Provided is a method for improving a food, wherein a &#x3b2;-amylase obtained fromis acted on a food containing a polysaccharide or an oligosaccharide having an &#x3b1;-1,4 bond of glucose as the main chain.

Method for improvement of foods utilizing &#x3b2;-amylase

The object is to provide a novel glycosyltransferase and the use thereof, the glycosyltransferase catalyzes transglucosylation of maltotriose units under conditions which can be employed for the processing of foods or the like. Provided is a maltotriosyl transferase which acts on polysaccharides and oligosaccharides having &#x3b1;-1,4 glucoside bonds, and has activity for transferring maltotriose units to saccharides, the maltotriosyl transferase acting on maltotetraose as substrate to give a ratio between the maltoheptaose production rate and maltotriose production rate of 9:1 to 10:0 at any substrate concentration ranging from 0.67 to 70% (w/v).

Polynucleotide encoding a maltotriosyl transferase

Maltotriosyl transferase and use thereof

A novel method of very efficiently elevating the yield of oligosacchrides containing &#x3b1;-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific &#x3b1;-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an &#x3b1;-galactosidase and thus performing a dehydrocondensation.

Method of elevating yield of oligosaccharides containing &#x3b1;-galactosyl and anti-candida compositions

This invention relates to a novel microorganism-derived enzyme having an activity to cut disaccharide glycosides (particularly, &#x3b2;-primeveroside and/or analogous disaccharide glycosides) in disaccharide unit, a method for producing the enzyme, a gene which encodes the enzyme and use of the enzyme. Various components can be formed by the action of this enzyme upon disaccharide glycosides.

Growing community of inventors

Kakamigahara, Japan

Masamichi Okada