Fujirebio Inc.

Fujirebio Kabushiki Kaisha

Fujizoki Pharmaceutical Co., Ltd.

Fujirebio Kabushiki Kaisha, Also Trading As Fujirebio Inc.

Masami Sugiyama

Yoshihiro Ashihara

Yasushi Kasahara

Mamoru Miyazaki

Yoshiaki Uchida

Tetsuji Tanimoto

Yoshihiro Kurano

Aiko Tanaka

Takahiro Harada

Hiroko Shibuya

The present invention relates to a fluorescent label characterized by containing a hydrophilic polymer having an anionic group, a polyether derivative, and a phosphor, in which the phosphor is bound to the hydrophilic polymer via the polyether derivative, and also relates to a fluorescently labeled recognition substance labeled with the fluorescent label, and an immunoassay method using the recognition substance.

Fluorescent label

Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen sulfide

Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.

Color developing method, enzyme immunoassay using the color developing method, and immunochromatography incorporating the enzyme immunoassay

Color developing method, enzyme immunoassay using the color developing

A method for measuring glycated hemoglobin is disclosed, which comprises contacting a sample with an anti-hemoglobin antibody being bound to a solid phase, adding thereto an anti-glycated hemoglobin antibody, and then measuring the anti-glycated hemoglobin antibody bound to the solid phase via glycated hemoglobin and the anti-hemoglobin antibody. A method for measuring glycated hemoglobin is further disclosed, which comprises mixing a sample with particles on which an anti-glycated hemoglobin antibody is bound to cause immunoagglutination reaction. A method for measuring glycated hemoglobin is furthermore disclosed, which comprises mixing a sample with a solution containing a monoclonal antibody specific for glycated hemoglobin, and then measuring the change in the turbidity of the resulting mixture. A method is disclosed for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and an anti-glycated hemoglobin antibody, to cause immunoagglutination reaction. There is disclosed a method for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and particles on which an anti-glycated hemoglobin antibody is bound, to cause immunoagglutination reaction. Still furthermore, an anti-glycated hemoglobin monoclonal antibody having a high specificity for human glycated hemoglobin is disclosed.

Anti-glycated hemoglobin monoclonal antibody and method for measuring

A method of chemiluminescence assay of the activity of peroxidase is disclosed, which employes (i) luminol or isoluminol as a substrate, (ii) hydrogen peroxide, and (iii) at lease one compound, serving as an enhancer for the activity of peroxidase, selected from the group consisting of ##STR1## wherein R.sup.1 represents a hydroxyl group, an alkali metal salt of hydroxyl group (--OM, where M is an alkali metal), an amino group which may have a substituent, a halogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent; R.sup.2 and R.sup.3 each represent hydrogen or a halogen; R.sup.4 and R.sup.5 each represent hydrogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent, or an alkoxyl group having 1 to 4 carbon atoms, which may have a substituent; ##STR2## wherein R.sup.6 represents an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or an alkene group having 2 to 6 carbon atoms, which may have a substituent, R.sup.7 to R.sup.10 each represent hydrogen, an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or a halogen, X represents oxygen or sulfur, Y represents --CH-- or nitrogen, which may be the same or different, and Y' represents carbon or nitrogen, which may be the same or different.

Method of the chemiluminescence assay of the activity of peroxidase

A method of measuring lipid-bound sialic acid (LSA) which comprises, mixing a LSA separating agent comprising a polar solvent with a sample containing LSA and other sialic acid(s), separating the precipitate formed to obtain a supernatant, and measuring the sialic acid concentration of said supernatant by using an enzyme capable of acting on sialic acid in a lipid-bound state. When a sample is treated with the LSA separating agent comprising a polar solvent, various components in the sample such as protein-bound sialic acid and proteinous substances precipitate while the desired lipid-bound sialic acid remains in the supernatent. Accordingly, by measuring sialic acid concentration of the supernatent, LSA concentration of the sample is easily determined. The method of the invention is simple and superior in reproducibility. The method of the invention is suitable for treating a large number of samples, and it can easily by automated. This method is suitable as a screening test for cancer.

Method of measuring lipid-bound sialic acid

A novel amylase inhibitor, obtained from hard wheat and durum wheat by using dye ligand affinity chromatography having an inhibitory activity on human salivary amylase of less than 0.1 of the inhibitory activity on human pancreatic amylase is disclosed which is useful for measuring isoenzymes of amylase in the field of clinical tests.

Amylase inhibitor and preparation and use thereof

Derivatives of dipeptide having the following general formula and their use as substrates for colorimetrically or spectrophotometrically measuring the activity of carboxypeptidase A: ##STR1## wherein X is OH in ortho or para position, or NH.sub.2 or CH.sub.3 O in para position, Y is ##STR2##

Derivatives of dipeptide and their use in determining the activity of

Derivatives of hippuryl-L-phenylalanine having the following general formula: ##STR1## wherein X represents OH or CH.sub.3 O useful as substrates for measuring the actively of carboxy peptidose A.

Derivatives of hippuryl-L-phenylalanine

A method for determining the activity of cholinesterase comprising the steps of:

Growing community of inventors

Tokyo, Japan

Masami Sugiyama