Mitsui Chemicals, Inc.

Meiji Seika Pharma Co., Ltd.

Masami Osabe

Kaoru Okakura

Fumikazu Yokoyama

Atsushi Inoue

Hisaaki Yanai

Hiroki Tamai

Toshihiro Oikawa

Nobuhiro Fukuhara

Setsuo Yoshino

Toshifumi Yamaki

Mitsuo Koito

Katsuyuki Takahashi

Hitoki Miyake

Teruo Arii

What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50&#xb0; C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.

Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose

This invention provides a novel D-aminoacylase obtained by cloning a DNA encoding the novel D-aminoacylase from Methylobacterium mesophilicum MT 10894, etc. and which shows sufficiently high activity at an industrially useful substrate concentration to allow a D-amino acid to be efficiently produced from an N-acyl-DL-amino acid; a DNA encoding the D-aminoacylase; a process for producing a D-amino acid from the corresponding N-acylamino acid using a transformant containing the DNA.

DNA encoding novel D-aminoacylase and process for producing D-amino acid by using the same

A purified N-t-butoxycarbonylphenylalanine ester preparation having an enhanced optical activity can be obtained by bringing an N-t-butoxycarbonylphenylalanine ester preparation containing an optically active compound into contact with an aliphatic hydrocarbon, extracting the optically active compound with the aliphatic hydrocarbon, and recovering the optically active compound from the resulting extract.

Process for preparing N-t-butoxycarbonylphenylalanine ester

Growing community of inventors

Chiba, Japan

Masami Osabe