Hitachi, Ltd.

Masako Mizuno

Yutaka Misawa

Kenji Tochigi

Yoshio Watanabe

Junkichi Miura

Taro Nogami

Yoshinori Takata

Hiroyuki Miyagi

In a liquid chromatographic system for analyzing biological or vital components in blood, etc., a separation column packed with a packing material of organic porous material containing carboxyalkyl groups as functional groups and having pore diameter of 600 to 1,200 .ANG. in dry state and an ion exchange capacity of 0.1 to 0.5 meq/g on dry basis is used to improve the chromatographic performance of the separation column, 5-component and 6-component analysis are carried out by switching separation columns of different column length or diameter, and judgement of column life is made by evaluation parameters.

Liquid chromatographic system and operation method

In separation column 23 is packed a filler consisting of an inorganic or organic porous substance having carboxyalkyl group and dihydroxyboronyl group. Thus, the separation column 23 can concurrently perform the procedure of separating glycohemoglobin, hemoglobin and hemoglobin derivatives in blood and the procedure of decomposing labile-type hemoglobin in blood into glucose and hemoglobin to remove the labile-type hemoglobin, when a diluted blood sample on sample table 19 along with eluents 8 to 10 is transferred to the separation column 23. Then, stable-type hemoglobin in the eluate from the separation column 23 is measured by UV-VIS detector 25.

Apparatus for measuring glycohemoglobin

Stable hemoglobin A.sub.lc (s-A.sub.lc) can be separated from other hemoglobin components and quantitated in a short time by means of a liquid-chromatographic apparatus comprising a separation column packed with a packing material consisting essentially of a porous substance having a carboxyalkyl group combined thereinto, as ion exchanger, a means for injecting a sample into the separation column, a means for passing one or more eluents through the separation column, and a means for detecting hemoglobin, hemoglobin derivatives or glycosylated hemoglobin, the average particle size of the packing material in dry state being 4 .mu.m or less.

Apparatus for liquid chromatography for separating AIC components from

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Mito, Japan

Masako Mizuno