Hitachi-high-technologies Corporation

Hitachi High-tech Corporation

Masako Kawarai

Toshinari Sakurai

Akihiro Shimase

Kazumichi Imai

Eiichiro Takada

Sadamitsu Aso

Shinya Ito

Makoto Nogami

Junkichi Miura

Daisuke Akieda

Hiroyuki Koshi

Iwao Suzuki

Izumi Ogata

Tami Hiruta

Yoshinori Inoue

The present invention makes it possible to realize an analysis apparatus having a plurality of liquid chromatographs capable of judging separation performance and the like at the right timing and improving analysis performance early. Analysis starts and a mixed sample is prepared by adding a non-retaining ingredient to a measurement object sample and is introduced into an analysis flow path (Sto S). The mixed sample is separated into components by a separation column, the components are outputted as chromatogram data by a detector, and the analysis finishes (Sto S). Retention time and peak information are acquired from the chromatogram outputted from the detector, whether or not a measurement result is within an allowable range is judged, and the process shifts to next analysis when the measurement result is within the allowable range (Sto S). An amount of transit time tof a non-retaining ingredient shifted from an allowable value is confirmed when separation performance is outside an allowable range at step Sand a separation column replacement instruction is outputted to an output unit when fluctuation of the transit time tis within an allowable range (S, S). A device maintenance instruction is outputted to the output unit when the fluctuation is outside the allowable range at step S(S).

Analysis apparatus having a plurality of liquid chromatographs and its analysis method

The present invention addresses the problem of providing: a cell dispersion measurement mechanism whereby it becomes possible to fully disperse cells regardless of the experiences of operators skilled in cell culture and it also becomes possible to determine the number or concentration of cells accurately; a cell culture apparatus equipped with the cell dispersion measurement mechanism; and a cell dispersion measurement method. The problem can be solved by circulating a cell suspension in a flow path to disperse cell masses contained in the cell suspension, and then determining over time the number or concentration of cells and/or the degree of dispersion of cells in the cell suspension that is flowing in the circulation flow path.

Cell dispersion measurement mechanism, and cell subculture system utilizing same

A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.

Cytometric mechanism, cell culture device comprising same, and cytometric method

A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.

Cell concentration adjustment device, and automatic subculture system using same

An object of the present invention is to provide a method and an apparatus by which PCBs in insulating oil can be analyzed with high accuracy in a convenient, inexpensive, and rapid manner. The method for analyzing polychlorobiphenyls by measuring the concentration of polychlorobiphenyls in insulating oil comprises a step of, as pretreatment, bringing particles of a copolymer that contains divinylbenzene and a methacrylate organic monomer as monomer components into contact with insulating oil containing polychlorobiphenyls, so as to separate polychlorobiphenyls in insulating oil from oil content that is an impurity. The methacrylate organic monomer preferably has a diol type hydroxyl group.

Method and apparatus for analysis of poly (biphenyl chloride) in electrical insulating oil

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Tokyo, Japan

Masako Kawarai