Toyo Jozo Kabushiki Kaisha

Toyo Jozo Company, Ltd.

Masaki Takada

Shuzo Satoi

Shigeyuki Imamura

Satoshi Yaginuma

Mitsuo Hayashi

Masashi Awata

Hideo Misaki

Kazuo Matsuura

Mamoru Takahashi

Hideo Sakakibara

Hidehiko Ishikawa

Akira Asahi

Naoki Muto

Shigeru Ikuta

Tetsu Saito

Kunio Matsumoto

Tetsuo Matsuda

Masaru Otani

Kiyofumi Fukukawa

Eiichi Yoshino

Akira Yagi

Minoru Toriya

Fumitaka Inoue

Hitoshi Sagai

Naoki Mutoh

Kenji Kinoshita

Kimio Mizuno

Katsumi Asano

A novel bilirubin oxidase is provided having a substrate specificity for bilirubin but not to, at least, biliverdin, catechol and hemin and which catalyzes the enzyme reaction between two moles of bilirubin and one mole of oxygen to yield two moles of biliverdin and two moles of water. A process is also provided for the production of said novel bilirubin oxidase, which comprises cultivating a bilirubin oxidase-producing fungus of the genus Penicillium in a culture medium and collecting the bilirubin oxidase from the culture mixture. A method of determining the content of bilirubin in a sample to be analyzed is further provided which comprises contacting the sample with the bilirubin oxidase to convert bilirubin in the sample into biliverdin and determining the amount of biliverdin formed or the amount of oxygen consumed therefor, as well as a method for removing bilirubin contained in a sample to be analyzed, which comprises contacting the sample with said novel bilirubin oxidase.

Novel bilirubin oxidase which has a substrate specifity to bilirubin,

Glucose dehydrogenase having the following biochemial properties:

Glucose dehydrogenase and its production

A compound of the formula ##STR1## wherein R is .beta.-D-glucopyranosyl, .beta.-D-galactopyranosyl, 6'-O-acetyl-.beta.-D-glucopyranosy or 6'-O-acetyl-.beta.-D-galactopyranosyl, or a pharmaceutically acceptable salt thereof, has bronchodilator, cardiac activity, smooth muscle relaxant activity and hormone excretion stimulant activity. It can be produced by culturing a microorganism belonging to the genus Nodulisporium and adapted to produce the compound in a culture medium, and isolating the produced compound from the cultured medium. The microorganism can be Nodulisporium sp. M5220 FERM P-8133. The compounds have inhibitory activity on cyclic-adenosine-3',5'-monophosphate phosphodiesterase.

Physiologically active substance TPI and production thereof

A physiologically-active novel substance 'aldostatin' having an estimated molecular formula of C.sub.20 H.sub.20 N.sub.2 O.sub.8 is produced by culturing an aldostatin-producing microorganism of the Pseudeurotium, for example, the Pseudeurotium zonatum M4109 strain. Aldostatin inhibits the aldose reductase activity and as a consequence, avoids abnormal accumulation of sorbitol, galactitol, etc. It is therefore effective for the treatment of chronic complications such as cataract, retinopathy and neuropathy caused by diabetes renalis.

Physiologically-active novel substance 'Aldostatin' and production

A substance is provided which has strong enzyme inhibitory activity against thiol proteases such as papain, ficin, bromelain, etc. and which is represented by the following formula: ##STR1## (wherein R is a hydrogen atom or a hydroxyl group), its pharmaceutically acceptable salt or hydrate.

Enzyme Inhibiting substance

Antibiotic acmimycin of the formula ##STR1## is active against Gram positive and Gram negative bacteria. It is produced by culturing the microorganism Streptomyces sp. AC4559 FERM P-6445, and isolating the above compound from the cultured medium.

Antibiotic acmimycin and its production

Antibiotic saccharocins of the formula ##STR1## wherein R is hydrogen (saccharocin) or hydroxyl (3'-oxysaccharocin) are produced by culturing a microorganism belonging to the genus Saccharopolyspora, for example Saccharopolyspora sp. AC 3440 FERM-P No. 6238, in a conventional nutrient medium, and separating the thus-produced saccharocins from the cultured medium. The noval saccharocins exhibit anti-bacterial activity against Gram negative bacteria.

Aminoglycoside antibiotic saccharocins

A process for the production of amine oxidase, which comprises culturing an amine-oxidase-producing microorganism belonging to the genus Talaromyces, genus Eupenicillium, genus Petromyces, genus Neosartorya or genus Eurotium, and isolating the thus-prepared amine oxidase from the cultured medium. The preferred strains of microorganisms under these genera are Talaromyces flavus var. flavus M 4175 FERM-P No. 5866, Eupenicillium parvum M 5051 FERM-P No. 5870, Petromyces alliaceus M 4648 FERM-P No. 5867, Neosaytorya fischeri M 4690 FERM-P No. 5868 and Eurotium chevalieri M 4805 FERM-P No. 5869.

Process for the production of amine oxidase

L-amino acid oxidase is obtained by culturing a microorganism which can produce L-amino acid oxidase and belongs to the genus Colletotrichum, e.g. Colletotrichum sp. M5073, deposition number FERMP 5441, in a culturing medium and recovering the oxidase from cells of the microorganism.

Process for producing L-amino acid oxidase

A process for the production of acyl-Coenzyme A oxidase, comprises culturing an acyl-Coenzyme A-oxidase-producing microorganism belonging to genus Macrophomina, genus Cladosporium, genus Aspergillus, genus Monascus, genus Saccharomyces or genus Arthrobacter in a nutrient medium, and isolating the thus-formed acyl-CoA oxidase therefrom. The preferred species of microorganism are Macrophomina phaseoli ATCC 14383, Cladosporium resinae IFO 6367, Aspergillus candidus M-4815 FERM-P No. 5226, Monascus sp. M-4800 FERM-P No. 5225, Saccharomyces cerevisiae Y 0036 FERM-P No. 5174, and Arthrobacter sp. B-720 FERM-P No. 5224, respectively.

Process for the production of acyl-Coenzyme A oxidase

Antibiotics aculeacin-A.alpha., -A.gamma., -D.alpha. and -D.gamma. are active against yeasts and fungi. They are produced by culturing the microorganism Aspergillus aculeatus NRRL 11270 in a nutrient medium containing assimilable carbon and nitrogen sources.

Antibiotic aculeacin-A.alpha., -A.gamma., -D.alpha. and -D.gamma. and

A family of antibiotics active against pathogenic yeasts and fungi comprises aculeacin-A, -B, -C, -D, -E, -F, and -G. These are produced from cultured broth of Aspergillus aculeatus FERM-P 2324.

Growing community of inventors

Shizuoka, Japan

Masaki Takada