The Institute of Physical and Chemical Research

Masaki Nojiri

Isao Endo

Masafumi Yohda

Masafumi Odaka

There is disclosed a method for producing nitrile hydratase comprising culturing a transformant cell which is obtained by transforming a host cell of bacterium not belonging to the genus Rhodococcus with a vector (1) which contains a nucleotide sequence encoding at least the &agr; a chain and the &bgr; chain of nitrile hydratase, or the vector (1) and a vector (2) which contains a nucleotide sequence encoding at least the &bgr; chain of the nitrile hydratase, and collecting produced nitrile hydratase, wherein the culture of the transformant cell is performed at a temperature of 35&deg; C. or less.

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Tsurugashima, Japan

Masaki Nojiri