Ajinomoto Co., Ltd.

Masakazu Sugiyama

Kunihiko Watanabe

Kenzo Yokozeki

Shunichi Suzuki

Shigeru Kawahara

Kenichi Mori

Tadashi Takemoto

Hiroyuki Nozaki

Yusuke Amino

Naoto Tonouchi

Tatsuki Kashiwagi

Nao Funakoshi

Ei-ichiro Suzuki

Yasuaki Takakura

Hidemi Fujii

Seiichi Hara

Yasuhiro Mihara

Sergey Vasilievich Smirnov

Veronika Aleksandrovna Kotliarova

Sonoko Suzuki

Toshiki Taba

Kenichi Hashiguchi

Uno Tagami

Shinji Kuroda

Eiichiro Suzuki

Natalia Nikolaevna Samsonova

Natalia Yurievna Rushkevich

Ken-ichi Mori

Yuki Imabayashi

Maiko Mori

Eri Tabuchi

Tatyana Aleksandrovna Bachina

Hiroomi Ogino

Koki Ishikawa

Akira Otaka

Akira Shigenaga

Olga Sergeevna Beznoschenko

The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.

Coryneform bacterium and method for producing heterologous fusion proteins

An object of the present invention is to provide a method for producing a peptide thioester compound, a peptide hydrazide compound, and a peptide amide compound. The present invention provides a method for producing a peptide hydrazide compound or a peptide amide compound by using a compound represented by Formula (2): wherein R, R, R, and X are as defined in the specification, and using a hydrazine compound or an ammonia compound as a reaction reagent. The present invention also provides a method for producing a peptide thioester compound from the peptide hydrazide compound.

Method for producing peptide hydrazide, peptide amide, and peptide thioester

The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Method for producing monatin using an L-amino acid aminotransferase

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus, or

Recombinant polynucleotide, host cells containing the same and a process for producing a protein having aldolase activity

A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by mutating the amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:4.

Mutated D-aminotransferase and method for producing optically active glutamic acid derivatives using the same

A process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin is described. This process entails converting a substituted &#x3b1;-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same. 

Process of producing glutamate derivatives

The present invention relates to a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted &#x3b1;-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same. 

Process for producing glutamate derivatives

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Methods for making 3-indole-pyruvic acid from tryptophan using a tryptophan deaminase

Oxidase gene and method for producing 3-indole-pyruvic acid by utilizing the gene

The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an &#x3b1;-amino acid is reacted with an aldehyde to form a serine derivative:

Method for producing serine derivative and protein used for the same

Aldolase and production process of substituted &#x3b1;-keto acids

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxalacetic acid) by using a novel aldolase derived from the genus, or

A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.

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Kawasaki, Japan

Masakazu Sugiyama