Hamamatsu-Photonics K.k.

Kyoto University

Masakazu Katsumata

Ayano Takeuchi

Yuko Kobayashi

Yukiko Sato

Kimiko Kazumura

Takashi Koike

Hiroshi Satozono

Hiroshi Tsuchiya

Masataka Nishikawa

Junichi Imanishi

Ryosuke Mochizuki

Lid for a culture vessel

The present invention discloses a method for evaluating vitality of a plant including (a) a step of measuring a delayed luminescence of a leaf of each plant of a group of plants subject to evaluation to obtain data of delayed luminescence amount, and (b) a step of processing data of a plurality of delayed luminescence amounts thus obtained to evaluate a plant exhibiting a delayed luminescence amount not less than a preset upper limit threshold as an individual of poor growth or evaluate a plant exhibiting a delayed luminescence amount not more than a preset lower limit threshold as an individual of good growth, and a measurement system and an evaluation system used for the method.

Method for evaluating vitality of plant, and measurement system and evaluation system

The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.

Method for preparation of algal cells, and kit for evaluation of toxicity of chemical substance

A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay values to assay biological growth inhibition factors. A biological growth inhibition factor assay method that enables analysis of a wide range of inhibition factors in a short time is thereby realized.

Harmful substance evaluating method and harmful substance evaluation kit

An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample. An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.

Evaluation method for evaluating a state of a photosynthesis sample

The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.

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Hamamatsu, Japan

Masakazu Katsumata