Shionogi Company Limited

Masaji Nishimura

Shinji Nishimura

Junko Uo

Setsuko Nakanishi

Hiroshi Nishi

Kazuo Horiguchi

Shigeru Kaku

The monoclonal antibodies of the present invention makes it possible to separate basophils suitable for the IgE-mediated specific chemical mediator release test, because it retains its reactivity with basophils even after being immobilized onto a solid carrier, and because it does not inhibit release of chemical mediators induced by allergens or anti-IgE antibody, and does not induce nonspecific release of chemical mediators. Also, the method for separating basophils of the present invention simplifies the separation of basophils from blood, and by using this method, the histamine release test which otherwise requires complex procedures can be simplified. Further, the group of cells obtained by the method for separating basophils of the present invention can easily be utilized in the release tests for chemical mediators released from basophils such as leukotriene and PAF, which otherwise require expertise for handling.

Basophil-binding monoclonal antibody, method for separation of

An assay for an immunologically active substance (B) in a biologically derived test sample comprising using an immuno-active membrane of a hydrophobic polymer sheet having numerous hydrophilic spots, on which a prefixed amount of an immuno-active substance (A) or (B) has been immobilized, and a coloring-membrane having numerous spots which contain a color-producing reagent being capable of developing color by the catalytic effect of an enzyme at the corresponding positions to those of the immuno-active membrane, and making contact the immuno-active membrane with said test sample in the presence of an enzyme-labeled immuno-active substance (A') or (B') and a substrate for reacting the substance (A), substance (B) and enzyme-labeled substance (A') or (B') competitively or non-competitively, and superposing the coloring-membrane of on the immuno-active membrane, followed by colorimetrically measuring the resulting color and thereby determining the amount of the substance (B) in the test sample.

Multi-spot enzyme immunoassay method

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Kyoto, Japan

Masaji Nishimura