Osaka University

Masahito Yamanaka

Katsumasa Fujita

Satoshi Kawata

Shogo Kawano

Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (), an objective lens () that focuses light from the laser light source on a sample, and a detector () that detects the laser light as signal light from a sample () when the sample () is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector () detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.

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Osaka, Japan

Masahito Yamanaka