Olympus Corporation

Masahito Dohi

Kenji Kawasaki

Kazuhiko Hosono

Yoshihiro Shimada

Atsuhiro Tsuchiya

Makio Ueno

Motohiro Shibata

Hiroyasu Hebiishi

An image processing device is configured to: generate a histogram of pixel values of a plurality of pixels contained in an image; set a background pixel value by using a peak value of the generated histogram; set a noise range with respect to the set background pixel value; and replace the pixel values that fall in the set noise range with a single arbitrary pixel value.

Image processing device, microscope system, image processing method, and image processing program

An observation device includes: an illumination unit that generates illumination light to be radiated onto cells floating in a culture fluid inside a culture vessel; a light receiving unit that receives observation light coming from the cells that have been irradiated with the illumination light, the observation light being imaged by an image-forming optical system; and a casing that has a transmissive section so as to transmit the illumination light and the observation light and that accommodates the illumination unit and the light receiving unit, wherein the casing has an elongated cylindrical form that is configured to be inserted into the culture fluid via a port used to insert a tube into the culture vessel.

Observation device

An observation device includes an objective lens disposed below a container to collect light from a specimen; a surface light source that is disposed at a pupil position of the objective lens in the optical path of the illumination light, that causes illumination light to enter the container from below, and that can change a light emission pattern in a direction intersecting an emission optical axis, an imaging optical system that captures light from the specimen generated by the specimen being irradiated with the illumination light from the surface light source and focused by the objective lens below the container; and a controller that corrects a light emission pattern on a basis of the light emission pattern and at least one of a brightness, contrast, and the relationship between the number of pixels and the luminance of an acquired image with the light emission pattern.

An illumination device includes: an objective; a first scanning device that moves a focusing position of light in an optical-axis direction of the objective; and a relay optical system that is configured to correct an aberration that is generated in the objective by the first scanning device moving the focusing position, the relay optical system being arranged on an optical path between the first scanning device and the objective.

Illumination device and microscope device

A microscope illumination device includes a white LED light source and an illumination optical system. The white LED light source includes a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light. The illumination optical system includes a field stop and a light diffusion element that is arranged between the white LED light source and the field stop. The microscope illumination device satisfies0.2&#x3c;&#x3c;1&#x2003;&#x2003;(1)

Microscope illumination device and microscope

A microscope apparatusincludes a detection optical systemthat captures light from a sample S and an illumination optical systemthat radiates an illumination light onto the sample S. The illumination optical systemincludes a cylindrical lensthat has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction, a cylindrical lensthat has a power in the second-axis direction and does not have a power in the first-axis direction, and a scannerthat scans the illumination light in a width direction. The illumination optical systemis configured such that the first-axis direction is the width direction described above, and the cylindrical lensesandare arranged posterior to the scanner

Microscope apparatus

In the present invention, cells cultured inside the wells of a microplate are observed easily and clearly regardless of the height or curvature state of the liquid surface of the culturing liquid. Provided is an observation device that includes an illumination optical system that irradiates a transparent sample with illumination light from a light source, an objective lens that collects observation light from the sample, a detection optical system that detects the observation light collected by the objective lens, and a retroreflective member that is arranged opposite the objective lens with the sample interposed therebetween and in which a plurality of very small reflective elements are arrayed. The objective lens and the illumination optical system are arranged below the sample in the direction of gravity.

A fluorescence observation unit includes a scanner that scans ultrashort pulsed laser light, a pupil projection lens that focuses the scanned ultrashort pulsed laser light, an image-forming lens that converts the focused ultrashort pulsed laser light to substantially collimated light and causes the ultrashort pulsed laser light to be incident on the objective lens, and a dichroic mirror that splits off, from the optic path of the ultrashort pulsed laser light, fluorescence generated in a sample due to irradiation with the ultrashort pulsed laser light and collected by the objective lens. The image-forming lens includes a first optical system having positive refractive power, and a second optical system having negative refractive power and disposed at a position closer to the scanner than the first optical system is. The dichroic mirror is disposed between the first optical system and the second optical system.

Fluorescence observation unit and fluorescence observation apparatus

A microscope illumination apparatus includes an optical conversion unit that converts laser light incident thereon from a light source to laser light that has an NA and whose main optical axis is distributed on a conical surface that widens at a predetermined angle with respect to an incident optical axis of the laser light, and from which the laser light exits, and an optical device that has no refractive power and that deflects the laser light, which has exited the optical conversion unit, in a direction parallel to the incident optical axis, wherein the microscope illumination apparatus focuses laser light that has exited the optical device at a pupil position of a focusing lens for illuminating a specimen or a position that is conjugate with the position.

Microscope illumination apparatus, microscope, and microscope illumination method

For achieving an expanded observation range without sacrificing resolution, a scanning optical system according to the present invention includes a scanner that deflects laser light from a light source while changing a deflection angle of the laser light; a polarization beam splitter that is capable of splitting-off the laser light from an optical path of the laser light deflected by the scanner; a polarization beam splitter that is disposed between the light source and the scanner and that causes the laser light split-off by the polarization beam splitter to travel toward the scanner; and a first relay optical system that has 1&#xd7; relay magnifying power and that is disposed between the polarization beam splitter and the polarization beam splitter. The first relay optical system relays the laser light split-off by the polarization beam splitter so as to cause the laser light to be incident again on the scanner at the same position as an incident position of the laser light from the light source via the polarization beam splitter.

Scanning optical system and scanning device

A collimator lens is configured with a double convex single lens formed with a resinous material, for collimating a light flux emitted from a light source. At least one of surfaces of the collimator lens is formed as an aspherical surface. The collimator lens satisfies following conditions:2&#x3c;|1/2|&#x3c;10;NA&#x3e;0.6,where Ris a curvature radius of a first surface, which is a lens surface on a side of the light source of the collimator lens, Ris a curvature radius of a second surface, which is a lens surface on a side opposite to the first surface of the collimator lens, and NA is a numerical aperture on the side of the light source of the collimator lens.

Double convex collimator lens, illumination device, and microscope

A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.

Microscope adapter unit

An illumination apparatus includes a light source and a collimator optical system converting light emitted from the light source to approximately-parallel light. The collimator optical system includes, in an order of proximity to the light source, a first lens having a positive power and including an approximately-flat surface and an aspheric surface, and a second lens having a positive power.

Illumination apparatus and microscope having the same

An illumination optical system which irradiates a sample surface with light through an illuminating lens includes: a light source; a condensing optical system receiving the light emitted from the light source; and a lens array optical system including a first lens array surface and a second lens array surface each formed by a plurality of lens elements. The first lens array surface has a conjugate relation with a back focal position of the illuminating lens. The second lens array surface is placed at a back focal position of the lens array optical system, has a conjugate relation with a pupil position of the illuminating lens, and the light source and the condensing optical system are arranged to form an image of the light source on the first lens array surface.

Growing community of inventors

Tokyo, Japan

Masahito Dohi