Toyo Boseki Kabushiki Kaisha

Masahiro Takagi

Tadayuki Imanaka

Masaaki Morikawa

Yoshihisa Kawamura

Masao Kitabayashi

Bunsei Kawakami

Hiroaki Inoue

Taku Arakawa

Hideyuki Komatsubara

Hideki Kamimura

A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3&prime;-5&prime; exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

Kit for amplifying nucleic acid

DNA encoding a thermostable DNA polymerase

A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

Methods of amplification using a thermostable DNA polymerase from the

Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1

A modified thermostable DNA polymerase having 5% or less of the 3'-5' exonuclease activity of the enzyme before modification and a DNA polymerase composition for amplifying nucleic acid, which comprises the modified thermostable DNA polymerase having 0 to 5% of the 3'-5' exonuclease activity of the enzyme before modification and an unmodified thermostable DNA polymerase having 3'-5' exonuclease activity or a modified thermostable DNA polymerase having 100 to 6% of the 3'-5' exonuclease activity of a thermostable DNA polymerase before modification; a method for amplifying nucleic acid by use of said modified thermostable polymerase or said DNA polymerase composition; and a reagent therefor.

Modified thermostable DNA polymerase derived from pyrococcus sp. KOD and

Growing community of inventors

Suita, Japan

Masahiro Takagi