Canon Kabushiki Kaisha

Japan Science and Technology Agency

Jeol Ltd.

Masahiro Shirakawa

Tetsuya Yano

Fumio Yamauchi

Yasuhiro Aoyama

Kimihiro Yoshimura

Shinsuke Sando

Katsuaki Kuge

Masato Minami

Hidetoshi Tsuzuki

Shigenori Tsuji

Tetsuya Matsuda

Teruyuki Kondo

Hidehito Tochio

Hisatsugu Yamada

Yoshinori Hasegawa

Yu Kimura

Yohsuke Yoshinari

Shingo Sotoma

Ryuji Igarashi

Yoshie Harada

Tetsuro Kobubo

Fuminori Sugihara

As a substance used as a contrast agent for a method of nuclear magnetic resonance analysis or a method of magnetic resonance imaging, a substance with high selectivity and high sensitivity was demanded. According to the present invention, when a polymer having, in a side chain thereof, a sequence of aH&#x2014;C&#x2014;N,H&#x2014;N&#x2014;C orH&#x2014;C&#x2014;C bond, that is, a structure labeled with stable isotopes ofC andN, is used, the abundance of such a sequence in one molecule can be increased, and hence, high selectivity and higher sensitivity can be attained when used as a contrast agent.

Polymer, contrast agent for nuclear magnetic resonance analysis or magnetic resonance imaging using the polymer, compound and method of nuclear magnetic resonance analysis and method of magnetic resonance imaging using the polymer

A nanodiamond particle including an NV center having ODMR intensity enhanced, of which surface is modified with a functional group containing a heteroatom, is provided. This nanodiamond particle as being chemically modified can serve for a fluorescent molecular probe which can be made use of in a biological system. By tracking a rotational motion of the NV center included in this fluorescent molecular probe, structural change of a protein can be analyzed in real time. The functional group containing a heteroatom can be at least any functional group of a hydroxyl group and a hydroxyalkyl group, or a carboxyl group.

Nanodiamond particle and method of manufacturing the same, and fluorescent molecular probe and method of analyzing structure of protein

An object of the present invention is to provide a substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.

Method of measuring enzyme activity by multi-dimensional nuclear magnetic resonance

The present invention provides a method for measuring nuclear magnetic resonance that employs a compound in which a plurality of nuclei is labeled with isotopes as a probe agent, highly selectively and highly sensitively obtains a nuclear magnetic resonance signal of the above described probe agent, and can attach a spatial positional information to the above described nuclear magnetic resonance signal, and an apparatus therefore.

Nuclear magnetic resonance measuring method using an isotope-labeled compound

Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.

Substrate probe for use in measuring enzyme activity

A magnetic resonance force microscope (MRFM) generator for producing an RF magnetic field uniformly over the whole of a sample. A cantilever with magnetic probe tip is self-excited. Under this condition, spins in the sample are controlled to produce a magnetic resonance force. A frequency demodulator measures the resonant frequency of the cantilever from the output detection signal from a cantilever displacement-measuring instrument based on the magnetic resonance force.

Magnetic resonance force microscope

Growing community of inventors

Kyoto, Japan

Masahiro Shirakawa