Sds Biotech K.k.

National Agriculture and Food Research Organization

Toyama Prefecture

Incorporated Administrative Agency National Agriculture Agriculture and Food Research Organization

Masahiro Oshima

Akihiko Yamazaki

Sakiko Hirose

Motoshige Kawata

Keisuke Sekino

Yuji Yamada

Hiroshi Kato

Yuzuru Tozawa

Hitoshi Yoshida

Satomi Takei

Makiko Kawagishi

Yoshihiro Sunohara

Yojiro Taniguchi

Hideo Maeda

Kazumasa Murata

Ikuo Ando

Hiroaki Maeda

In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Method for producing HSL protein having improved catalytic activity for 2-oxoglutaric acid-dependently oxidizing 4-HPPD inhibitor

By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.

Plant having increased resistance or susceptibility to 4-HPPD inhibitor

Growing community of inventors

Tsukuba, Japan

Masahiro Oshima