Mitsubishi Denki Kabushiki Kaisha

Mitsubishi Electric Semiconductor Software Co., Ltd

Jcr Pharmaceuticals Co., Ltd.

Masahiro Asano

Kimikatsu Matsubara

Atsuko Kawasaki

Atsushi Sugimura

Kazutoshi Mihara

Yoshiki Cho

Tsuyoshi Fukui

Yukichi Hatano

Toshihiro Yagi

Masayuki Utsuno

Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.

Method for production of recombinant human alpha-galactosidase A

The oscillation-stop detecting device accurately detects the stopping of a clock signal due to various causes. The voltage detecting circuit detects when a clock signal output from the clock signal oscillator remains at a high, a low or an intermediate level, and outputs a clock voltage detection signal. The oscillation-stop detecting circuit outputs a detection signal in response to the voltage detection signal.

Device for detecting a plurality of signal levels indicating stopping of

Even in the case where a microcomputer according to the present invention is directly connected to a bus in a LAN, it is possible to upgrade the data transfer speed of the LAN. When a built-in exclusive-OR circuit in the SIO of the microcomputer detects the discordance between a signal at an R.times.D terminal and a signal at a T.times.D terminal, a D flip-flop circuit generates and sends out an interrupt signal to the CPU. The CPU is made to recognize the collision of signals by the generation of the interrupt signal.

Microcomputer with built-in serial input-output circuit and collision

Growing community of inventors

Hyogo, Japan

Masahiro Asano