Nippon Kayaku Kabushiki Kaisha

Masahiko Yabuuchi

Minoru Masuda

Hiroshi Akanuma

Tsuneo Nakamura

Masashi Hashiba

Shigeru Tajima

Hiroshi Hayami

Reiko Machida

Yoshihiko Umegae

Kazuo Kato

Kazuo Katoh

Masachika Hirayama

Tomoko Takezawa

Akira Takahashi

Yayoi Irie

Mamoru Kunishima

Hideki Yoshioka

Kikuo Imazumi

Hiroaki Hamano

Shuhei Tsukamoto

Akinori Naito

Umegae Yoshihiko

Irie Yayoi

Machida Reiko

The invention is a method for determining the concentration of an analyte in whole blood, wherein the analyte is a component which is contained in the blood, is different from a component occurring only in a red blood cell, and can generate hydrogen peroxide upon the reaction with an oxidase. Whole blood is utilized in the method. The method comprises the steps of hemolyzing the whole blood and detecting hydrogen peroxide generated by the reaction between the analyte and the oxidase. The measurement method can avoid the inhibition of color development by hemoglobin and the interference with the measurement by hemoglobin. Further it can be used for biological tests that are carried out in a household, an individual doctor's clinic or at the bedside of patients without the need for any blood cell separation procedure or the like, because the measurement utilizes whole blood.

Blood component measurement method utilizing hemolyzed whole blood, and kit for the method

A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.

Thermostable 1,5-anhydroglucitol dehydrogenase, and method for measurement of 1,5-anhydroglucitol by using the same

A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaing the sugar-alcohols in the effluent out of the column, a column filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.

Method for quantitatively measuring sugar-alcohol, column and kit

This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to:

Method for assaying 1,5-anhydroglucitol and kit therefor

The present invention relates to a method of quantitative assay for 1,5-anhydroglucitol which comprises oxidizing 1,5-anhydroglucitol in an aqueous solution of a specimen in the presence of an electron acceptor to produce a compound represented by formula (1) below or a hydrate thereof represented by formula (2) below and quantitatively determining 1,5-anhydroglucitol from a consumption amount of said electron acceptor in said aqueous solution of a specimen or from a production amount of the reduction product of said electron acceptor produced or from an amount of oxidized product of 1,5-AG represented by formula (1) or formula (2). ##STR1##

Method of quantitative assay for 1,5-anhydroglucitol

Disclosed are an N-benzenesulfonyl derivative of cephamycin C represented by the following formula: ##STR1## which can easily be separated as a solid from an aqueous solution thereof, and a process for the preparation thereof. This derivative is valuable as an intermediate leading to a cephamycin type antibiotic substance.

Derivative of cephamycin C

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Takasaki, Japan

Masahiko Yabuuchi