Sanbio, Inc.

Masahiko Takano

Mari Dezawa

Hajime Sawada

Hiroshi Kanno

There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which method comprises introducing a Notch gene and/or a Notch signaling related gene into the cells, wherein the finally obtained differentiated cells are the result of cell division of the bone marrow stromal cells into which the Notch gene and/or Notch signaling related gene have been introduced. The invention also provides a method of inducing further differentiation of the differentiation-induced neural cells to dopaminergic neurons or acetylcholinergic neurons. The invention yet further provides a treatment method for neurodegenerative and skeletal muscle degenerative diseases which employs neural precursor cells, neural cells or skeletal muscle cells produced by the method of the invention.

Neural precursor cells expressing exogenous Notch intracellular domain

Treatment of CNS disorders using descendants of MSCS expressing an exogenous Notch intracellular domain

Treatment of CNS disorders using descendants of MSCs expressing an exogenous Notch intracellular domain

Neural precursor cells produced from bone marrow stromal cells containing an exogenous nucleic acid encoding a notch intracellular domain (NICD)

Method of inducing differentiation of bone marrow stromal cells to neural precursor cells, neural precursor cells, and uses thereof

Bone marrow stromal cells comprising a nucleic acid encoding a notch intracellular domain

Differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene

There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and/or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.

Schwann cells originating in myeloid interstitial cells

Growing community of inventors

Yokohama, Japan

Masahiko Takano