Showa Denko K.k.

Masahiko Seko

Kiyoshi Saito

Eiko Masatsuji

A cellulase is provided having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus FERM BP-3431 or a mutant strain thereof. Also disclosed is a detergent comprising the cellulase, a builder and a surfactant. A composition and a method of treating a paper pulp slurry is also disclosed.

Composition containing celluase from Bacillus Ferm BP-3431 or a mutant

A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by a Bacillus strain FERM BP-3431 or a mutant strain thereof. A detergent comprising the cellulase, a builder and a surfactant is also disclosed.

Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a

A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus sp. SD402, its mutant strains or its genetic engineered strains. An aid for detergents and a paper treating agent, respectively, contain the cellulase as an effective ingredient.

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Tokyo, Japan

Masahiko Seko