Dai Nippon Printing Co., Ltd.

Masahiko Hase

Hideshi Hattori

Masatoshi Kuroda

Norihiko Okochi

Katsunori Tsuchiya

Kazumasa Yamaki

Taro Nagai

Yumiko Narita

Hideeshi Hattori

This invention provides a cost-effective technique for mass-production of a cell culture vessel that allows mass cell culture to be performed in a manner that allows cells to be stably detached from the culture vessel. Specifically, the invention provides a method for producing a cell culture vessel suitable for large-capacity culture comprising cutting a long-sized cell support film to obtain a sheet-like cell support film, fixing the film to a first member, which is not closed, and bonding other members thereto. Thus, a cell culture vessel comprising a container section in which a cell support film is fixed to an inner wall surface can be produced.

Method for producing cell culture vessel

This invention provides a method for obtaining biological tissue having a three-dimensional structure of interest. The method of the invention comprises: adding a cell-containing culture solution to a culture vessel having an inner bottom surface, which is non-cell adhesive and comprises a concave-convex pattern provided thereon; performing cell culture under conditions in which intercellular adhesion takes place while centrifugal or magnetic force toward the inner bottom surface is applied to the cells that were added to the culture vessel to form tissue via intercellular adhesion; and detaching and collecting the resulting tissue from the inner bottom surface at the end in order to obtain tissue having a three-dimensional configuration using a concave-convex pattern as a template.

Method for preparing biological tissue

This invention provides a method for preparing biological tissue in which the thickness (i.e., the number of cell layers) can be easily regulated, culture can be conducted within a shorter period of time than is possible with conventional techniques, and addition of unfavorable components is not necessary. The method of the invention comprises adding a cell-containing culture solution to a culture vessel having a non-cell-adhesive inner bottom surface, conducting cell culture while centrifugal force toward the inner bottom surface is applied to the cells in the vessel, forming tissue via intercellular adhesion, and detaching and collecting the resulting tissue from the inner bottom surface.

The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method.

Test kit comprising a culture instrument with a cell pattern and a gel suitable to embed cell pattern

It is an object of the present invention to provide a cell culture carrier for producing a cell sheet that can be readily detached from a cell culture carrier and is inhibited from contracting after being detached. The cell culture carrierof the present invention comprises: a support-held culture membranecomprising an organic thin filmhaving cell adhesion properties and biodegradability and a frame-like supportfixed on the periphery of the organic thin film for maintaining the dimensions of the organic thin film; and a base substratehaving a surfacewith a static water contact angle of 45&#xb0; or less, wherein the support-held culture membraneis detachably placed on the surfaceof the base substrate.

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Tokyo, Japan

Masahiko Hase