Takeda Chemical Industries, Inc.

Masahide Oka

Takamasa Yamaguchi

Ikuo Nogami

Toshihiro Ishiguro

Hideo Shirafuji

Yoshiaki Uda

Yasushi Nakagawa

Kenkichi Yoneto

Takako Yamauchi

A composition is disclosed comprising a water-insoluble or slightly water-soluble compound and a branched cyclodextrin-carboxylic acid. The branched cyclodextrin-carboxylic acid significantly increases the water-solubility of the compound. Also disclosed is a method of enhancing water-solubility of the compound.

Composition containing a water-insoluble or slightly water-soluble

In a microbial process for producing 2-keto-L-gulonic acid (hereinafter sometimes referred to as 2KGA) by fermentation or microbial cell reaction with at least one microorganism capable of producing 2KGA, wherein the improvement comprises taking out, from the fermentation broth or microbial cell reaction mixture, the produced 2KGA alone or together with a low molecular-weight cation as the counter ion by electrodialysis.

Process for producing 2-keto-L-gulonic acid using electrodialysis

A process for producing a saccharide carboxylic acid or a salt thereof characterized in that a microorganism belonging to the genus Pseudogluconobacter and capable of oxidizing a hydroxymethyl group and/or hemiacetal hydroxyl-associated carbon atom to a carboxyl group, or an artifact derived from the microorganism, is permitted to act on a hydroxymethyl and/or hemiacetal hydroxyl-containing saccharide or saccharide derivative to produce and accumulate the corresponding carboxylic acid and the carboxylic acid so accumulated is harvested and novel saccharide carboxylic acids produced by the above production method, and by the process, from a broad range of saccharides, saccharic acids having carboxyl groups derived from hydroxymethyl and/or hemiacetal OH groups can be produced with high selectivity and in good yield, the resultant saccharide acids are resistant to enzymatic degradation and have improved water solubility, among other characteristics.

Production of saccharide carboxylic acids

Saccharide carboxylic acids of glycosides

2-keto-L-gulonic acid is in high yield produced by contacting a microorganism of the genus Pseudogluconobacter, either as it is or after processing, with L-sorbose.

Method for producing 2-keto-L-gulonic acid

L-sorbose, an intermediate important in vitamin C synthesis, is produced in higher yield from D-sorbitol by microbiological oxidation using a microorganism which belongs to the genus Gluconobacter and which has decreased ability to grow with D-sorbitol as the single carbon source compared with that of its parent strains.

Method for producing L-sorbose

An improved process for producing 2-keto-L-gulonic acid which comprises culturing a microorganism belonging to the genus Pseudogluconobacter which has an ability to oxidize L-sorbose to 2-keto-L-gulonic acid in a culture medium supplemented with a rare earth element in the presence of L-sorbose.

Growing community of inventors

Kawanishi, Japan

Masahide Oka