The Institute of Physical and Chemical Research

Masafumi Yohda

Isao Endo

Masafumi Odaka

Koji Takio

Masayoshi Nakasako

Masaki Nojiri

Disclosed are peptides represented by IVC SLC SC TAW and C SLC SC wherein I stands for isoleucine, V for valine, C for cysteine, C for cysteinesulfinic acid, C for cysteinesulfenic acid, S for serine, L for leucine, T for threonine, A for alanine, and W for tryptophan. These peptides can impart photoreactivity to a protein by binding to a non-heme iron. Also disclosed are methods for imparting photoreactivity to cells by introducing the peptide sequences into at least one protein which is involved in a metabolic system and/or energy metabolic system of the cells. There can be provided peptide sequences with a shorter peptide chain capable of imparting photoreactivity, which can be easily introduced into a protein with a little risk in degrading original function of the protein. There are also provided methods enabling control of metabolic reactions by imparting photoreactivity to cells with the peptides.

Undecapeptides containing cysteinesulfinic and cysteinesulfenic acid

There is disclosed a method for producing nitrile hydratase comprising culturing a transformant cell which is obtained by transforming a host cell of bacterium not belonging to the genus Rhodococcus with a vector (1) which contains a nucleotide sequence encoding at least the &agr; a chain and the &bgr; chain of nitrile hydratase, or the vector (1) and a vector (2) which contains a nucleotide sequence encoding at least the &bgr; chain of the nitrile hydratase, and collecting produced nitrile hydratase, wherein the culture of the transformant cell is performed at a temperature of 35&deg; C. or less.

Method for producing nitrile hydratase

Disclosed are peptides represented by IVC,SLC,SC,TAW and C,SLC,SC,wherein I stands for isoleucine, V for valine, C,for cysteine, C,for cysteinesulfinic acid, C,for cysteinesulfenic acid, S for serine, L for leucine, T for threonine, A for alanine, and W for tryptophan. These peptides can impart photoreactivity to a protein by binding to a non-heme iron. Also disclosed are methods for imparting photoreactivity to cells by introducing the peptide sequences into at least one protein which is involved in a metabolic system and/or energy metabolic system of the cells. There can be provided peptide sequences with a shorter peptide chain capable of imparting photoreactivity, which can be easily introduced into a protein with a little risk in degrading original function of the protein. There are also provided methods enabling control of metabolic reactions by imparting photoreactivity to cells with the peptides.

Growing community of inventors

Wako, Japan

Masafumi Yohda