Tohoku University

Japan Vam & Poval Co., Ltd.

Nipro Corporation

Tokyo University of Agriculture and Technology

Meiji Seika Pharma Co., Ltd.

Meui Seika Pharma Co., Ltd.

National University Corporation Tokyo University of Agriculture and Technology

Masafumi Goto

Yoshihiro Kimura

Akinobu Oharuda

Youhei Yamagata

Kimiko Watanabe

Yoshihiro Yoshikawa

Takayoshi Fukushima

Kengo Yokoyama

Kei Matsuo

Kazutaka Murayama

A cell or tissue embedding device having an aqueous gel serving as an immunoisolation layer, the aqueous gel containing, as components thereof, a denatured polyvinyl alcohol resin having an activated carbonyl group and a crosslinking agent is highly capable of supplying a physiologically active substance.

Cell or tissue embedding device

Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived fromsp. According to this method, the type and amount of protein-degrading enzyme to be used for isolating cells can be determined from the composition of a major protein of the biological tissue. Thus, cells having a high biological activity can be efficiently separated while reducing the amount of protein-degrading enzyme to be used.

Method for separating cell from biological tissue

An object of the present invention is to provide a method for preserving pancreatic islet, a container for preserving pancreatic islet and a kit for transplanting pancreatic islet in order to effectively preserve the pancreatic islet. The present invention presents; a method for preserving a liquid including pancreatic islet in a container for preserving pancreatic islet, wherein at least a part of the container wall face consists of a film having an oxygen permeation coefficient of 2500 cm/m&#xb7;day&#xb7;atm or greater; a method for preserving a liquid including pancreatic islet in a container for preserving pancreatic islet, wherein at least a part of the container wall face consists of a film having a carbon dioxide permeation coefficient of 1000 to 20000 cm/m&#xb7;day&#xb7;atm; and a container for preserving pancreatic islet for use in the above-described method, and a kit for transplanting pancreatic islet comprising the container for preserving pancreatic islet.

Method for preserving pancreatic islet, container for preserving pancreatic islet, and kit for transplanting pancreatic islet

The present invention relates to a method for obtaining cells or cell populations having a high biological activity from a biological tissue by enzymatic isolation, and probes for use in the method; more specifically to a method for analyzing a biological tissue, comprising applying two or more probes respectively containing biological-component binding domains through which two or more proteins bind to a predetermined biological component, to an isolated biological tissue, and analyzing binding amounts of the probes to the biological tissue.

Probe for analyzing biological tissue and method for utilizing same

Growing community of inventors

Sendai, Japan

Masafumi Goto