Ventana Medical Systems, Inc.

Mary Padilla

Christopher Bieniarz

Jerome W Kosmeder, Ii

Michael Patrick Farrell

Brian Daniel Kelly

Thomas M Grogan

Fabien Gaire

Hiroaki Nitta

Richard Gniewek

Megan Lehrkamp

Michael Farrell

Jerome Kosmeder

The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.

Method for chromogenic detection of two or more target molecules in a single sample

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Tucson, AZ, United States of America

Mary Padilla