Promega Corporation

Mary P Hall

Keith V Wood

Lance P Encell

Monika G Wood

Thomas A Kirkland

Thomas Machleidt

Poncho Meisenheimer

Brock Binkowski

Matthew B Robers

Joel R Walker

Anton Shakhmin

James Unch

Melanie Dart

Nidhi Nath

Marie Schwinn

Said A Goueli

Andrew S Dixon

Hicham Zegzouti

Dileep K Pulukkunat

Subhanjan Mondal

Wenhui Zhou

Gediminas Vidugiris

Paul Otto

Michael R Slater

Kristopher Zimmerman

Ce Shi

Michael Killoran

Rachel Friedman Ohana

Christopher Todd Eggers

Horacio Lazaro

Dieter Klaubert

John William Shultz

Harry Tetsuo Uyeda

Michael P Valley

Roderick G Flemming

Trish Hoang

Tamara Sue Pratt

Virginia Kincaid

Brock F Binkowski

Thomas Kirland

Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.

Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

-derived luciferases, novel coelenterazine substrates, and methods of use

Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

Substituted imidazo[1,2-α]pyrazines as luciferase substrates

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.

Multipartite luciferase peptides and polypeptides

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Compounds that may selectively inhibitluciferase-derived bioluminescent complexes, e.g., NanoBiT® bioluminescent complex, are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Inhibitors of oplophorus luciferase-derived bioluminescent complexes

Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I): by reacting a compound of formula (xi): with carbonyldiimidazole to form the compound of formula (I).

Process for preparing substituted imidazo[1,2-&#x3b1;]pyrazines

Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

Luciferase enzymes for use with thermostable luciferins in bioluminescent assays

Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Coelenterazine analogues

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Syntheticluciferases with enhanced light output

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.

Nanoluc suicide substrates

Activation of bioluminescene by structural complementation

Growing community of inventors

Madison, WI, United States of America

Mary P Hall