Purdue Research Foundation

Li-cor, Inc.

Mary Jean Wirth

Zhaorui Zhang

Robert E Birdsall

Yimin Hua

Michael D Furtaw

Jon P Anderson

Sampath Ranasinghe Kodithuwakkuge

Charu Yerneni

A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.

Protein chromatography matrices with hydrophilic copolymer coatings

A self-assembled engineered lattice of nanometer-scale silica particles, or other suitable particles generally resembling regularly-sized spheres, is configured in a separation bed for electrophoresis, isoelectric focusing, chromatography, or other voltage-induced separation of analytes. After separation, the analytes are immobilized on the separation bed and then ionized using matrix-assisted laser desorption/ionization (MALDI) for use with a mass spectrometer. The nanoparticles can be coated with polymers that activate to immobilize the analytes or assist with MALDI. The separation can occur in two dimensions.

Laser desorption ionization mass spectrometry using a particulate separation bed

Improved methods for packing a chromatographic column and columns packed by these methods are provided. The methods include introducing a packing material into a chromatographic column, while simultaneously ultrasonicating and applying pressure to the packing material to form a packed column with radial homogeneity.

Method of packing chromatographic columns

Growing community of inventors

West Lafayette, IN, United States of America

Mary Jean Wirth