Smithkline Beecham Corporation

Other

Mary E Brawner

Nabil A Elshourbagy

Paul G Lysko

Francis J Schmidt

James A Fornwald

Derk J Bergsma

Usman Shabon

Craig W Adams

Mary S Barnette

John A Keller

Robert G Gerber

Mary J Donovan

Edward J Arcuri

Mouse CC-CKR5 polypeptides and DNA (RNA) encoding such mouse CC-CKR5 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such mouse CC-CKR5 in the development of gene knockout mice for use as a model for human immunodeficiency virus.

Mouse CC-CKR5 receptor polypeptide

The invention provides a method of screening compounds to identify those which enhance or block the action of CC-CKR5. Further provided are compounds which blcok (antagonists) or enhance (agonists) the action of CC-CKR5.

Method of identifying agonists and antagonist for CC-CKR5 receptor

Attachment enhanced 293 cells

Attachment enhanced human embryonic kidney cells, 293, are provided. These cells have been modified to contain a selected mammalian scavenger gene, which has been found to improve the ability of these cells to attach in culture. The improved cells of the invention are useful in assays in which the unmodified 293 cells could be used.

A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter; P2 promoter; P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

Gal operon of streptomyces

A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter, P2 promoter, P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

A method is provided for improving the yield of the heterologous proteins sCD4 and derivatives of sCD4, produced by recombinant Streptomyces lividans by the addition of casamino acids to the nutrient medium in which the bacteria are cultured. Also provided by this invention is a method for improving the half-life of heterologous proteins in a cell free culture supernatant by the addition of casamino acids to the supernatant. Complex media are also provided.

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Berwyn, PA, United States of America

Mary E Brawner