Arcadia Biosciences, Inc.

Mary DePauw

Allen G Good

Ashok K Shrawat

Jean C Kridl

George Theodoris

Methods of increasing nitrogen utilization efficiency in monocot plants through genetic modification to increase the levels of alanine aminotransferase expression and plants produced there from are described. In particular, methods for increasing the biomass and yield of transgenic monocot plants grown under nitrogen limiting conditions compared to non-transgenic plants are described. In this way, monocot plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

Nitrogen-efficient monocot plants

Methods are provided by whichplants and seeds thereof may be modified to express a coding region of interest using a promoter sequence operatively linked to the coding region. The promoter sequence is an isolatedantiquitin (OsAnt1) promoter sequence including SEQ ID NO: 1. The coding region of interest may encode a nitrogen utilization protein, suitably alanine aminotransferase. Methods to developplants that have increased biomass and seed yield are also presented. Furthermore,plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

Growing community of inventors

Edmonton, Canada

Mary DePauw