Syngenta Participations Ag

Ciba-Geigy Corporation

Syngenta Crop Protection Ag

Novartis Finance Corporation

Washington University

The Penn State Research Foundation

Mary-Dell Matchett Chilton

Qiudeng Que

Heng Zhong

Zhongying Chen

Weining Gu

Myoung Kim

Yaping Jiang

Mark Scott Rose

Christian T Harms

Yin-Fu Chang

Gleta Carswell

Ailing Zhou

Yinping Lucy Qin

John H Duesing

Shujie Dong

Genevieve Hansen

Wenling Wang

Kenneth Allen Barton

Andrew Norton Binns

Antonius J Matzke

Janet Louise Suttie

Georgia Helmer

Steven Rothstein

Liliana Scarafia

Chen-Pei D Tu

Hui-Chen J Lai

Eric Levy

Yingping Lucy Qin

Mark Rose

The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.

Methods and compositions for targeted genomic insertion

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Targeted insertion sites in the maize genome

The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications

The invention provides three novel disarmed strains ofbacteria useful for the transformation of plants. The invention provides three engineeredChry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

strains

The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising: introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site; and introducing into the plant cell an integrase or integrase complex.

Lambda integrase mediated recombination in plants

The present invention provides an improved method for achieving stable integration of an exogenous DNA fragment in intact form into the genome of a eukaryotic cell, particularly a plant cell. The method comprises providing the exogenous DNA together with one or more proteins which promote integration of the exogenous DNA to the eukaryotic cell targeted for transformation, wherein the proteins are provided in the form of a chimeric gene or translatable RNA capable of expression in the eukaryotic cell. The method is particularly applied to plant cells to achieve stable integration of an exogenous DNA fragment bounded by T-DNA borders in intact form using integration-promoting proteins derived from Agrobacterium. Transgenic cultures, tissues and whole organisms, particularly transgenic plants, can be generated from cells transformed according to the method of the invention.

Method for achieving integration of exogenous DNA delivered by

Inactivation of the cytokinin autonomy gene of T-DNA in broad host range Ti plasmid produces mutant T-DNA vectors suitable for insertion of foreign genes; insertion of the mutant T-DNA by an in vitro tissue culture technique or any other technique into plant cells produces genetically engineered plant cells that can be regenerated into complete plants with roots. The inactivation of the cytokinin autonomy gene disarms the Ti plasmid and produces a useful gene vector for higher plants. The inactivation of the cytokinin gene may be accomplished by techniques such as point mutation, inversion, deletion, transposition, substitution or insertion. In the case of tobacco, for example, transformation of tobacco stem segments with engineered bacterial strains resulting from the insertion of DNA encoding yeast alcohol dehydrogenase and a bacterial neomycin phosphotransferase into the T-DNA of Agrobacterium tumefaciens plasmid pTiT37 at the 'rooty locus' produced transformed plant cells that were capable of regeneration into intact, normal tobacco plants. The yeast gene and entire T-DNA were present in the regenerated plants in multiple copies, and nopaline was found in all tissues. The plants were fertile and seedlings resulting from self-pollination also contained intact and multiple copies of the engineered T-DNA.

Regeneration of plants containing genetically engineered T-DNA

According to the invention there is provided a method of controlling insect larvae comprising the steps of feeding said larvae an insecticidal amount of a transgenic Zea mays plant that expresses a polypeptide having the insect toxicity properties of Bacillus thuringiensis crystal protein.

Method of controlling insect larvae comprising feeding an insecticidal

Methods for protecting ZEA mays plants against pest damage

The invention relates to plant cells and dicotyledonous plants transformed with chimeric rat glutathione-S-transferase gene constructs. Transgenic tobacco plants and progeny expressed the chimeric gene constructs under the control of a plant promoter. The transgenic plants and progeny demonstrated resistance to a herbicide which is normally inhibitory to plants not expressing the chimeric construct.

Herbicidal tolerant plants containing rat glutathione S-transferase gene

Growing community of inventors

University City, MO, United States of America

Mary-Dell Matchett Chilton