Kosan Biosciences Incorporated

University of California

Mary C Betlach

Robert G McDaniel

Li Tang

Gary W Ashley

Melanie C Betlach

Claes Gustafsson

Rainer Ziermann

George J Turner

Neal I Gutterson

Bryan Julien

James T Kealey

Ed Ralston

Sanjay Krishnakant Shah

Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.

Recombinant narbonolide polyketide synthase

Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for picromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method.

Polyketide synthase genes from Streptomyces venezuelae

Novel Sorangium polyketide synthases, and domains thereof, and polynucleotides encoding therefor. Additionally, chimeric polyketide synthases that include domains, or subsets of domains, patterned on said novel polyketide synthases. Methods to prepare polyketide combinatorial libraries are described, as are recombinant host cells in which polyketides are produced.

Sorangium polyketide synthases and encoding DNA therefor

The present invention provides genetically altered plants and plant cells that have been modified to contain expression system(s) capable of expressing a functional polyketide synthase (PKS). The present invention further provides methods of producing PKS and polyketides using these plants and cells.

Production of polyketides in plants

Recombinant DNA compounds that encode all or a portion of the oleandolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of oleandolide, oleandolide derivatives, and polyketides that are useful as antibiotics and motilides.

Recombinant oleandolide polyketide synthase

Yields of polyketides produced in host cells such as Streptomyces can be increased by coexpression of the ptpA gene. The introduction of recombinant vectors encoding ptpA and polyketide synthase genes is more efficient and does not require methyl-free DNA if the host cell is a restriction/methylation deficient strain, such as,K4-114, K4-155, or K27-39.

Recombinant host cells for the production of polyketides

Domains of epothilone polyketide synthase, and polynucleotides encoding therefor. Additionally, chimeric polyketide synthases that include domains, or subsets of domains, patterned on epothilone polyketide synthase. Methods to prepare epothilone in pharmaceutically useful quantitites are described, as are methods to prepare polyketide combinatorial libraries.

Sorangium polyketide synthase

This invention relates to the preparation and use of expression systems capable of producing heterologous polypeptides in halobacterial hosts.

Growing community of inventors

San Francisco, CA, United States of America

Mary C Betlach