Pioneer Hi-Bred International, Inc.

E.i. Dupont De Nemours and Company

The Goodyear & Tire Rubber Company

Danisco US Inc.

Mary Beatty

Gina Marie Zastrow-Hayes

Kevin Hayes

Jenna Hoffman

Haining Lin

J Antoni Rafalski

Jonathan E Lightner

James A Janni

Derek H Wells

Zhenglin Hou

Christopher Lee Rife

Gregory D May

Stephane Deschamps

Nicholas Doane Chilcoat

David J Meyer

Kishore Nannapaneni

Antoni J Rafalski

Kevin Robert Hayes

Jenna Lynn Hoffman

Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Methods for characterizing DNA sequence composition in a genome

Compositions and methods of identifying and characterizing potential gene editing on-target and off-target sites and/or edits in a nucleic acid are provided.

Methods of identifying and characterizing gene editing variations in nucleic acids

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyses of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

Using structural variation to analyze genomic differences for the prediction of heterosis

Methods for characterizing a target DNA sequence composition in a plant genome

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyzes of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

Using oligonucleotide microarrays to analyze genomic differences for the prediction of heterosis

The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.

Legume isoprene synthase for production of isoprene

Growing community of inventors

Earlham, IA, United States of America

Mary Beatty