Centre National De La Recherche Scientifique

Other

UniversitÉ De Lausanne

Institut National De La Recherche Agronomique

Institut National De La Recherche Agronomique (inra)

Azienda Socio Sanitaria Territoriale Papa Giovanni Xxiii

University of Lausanne

L'institut National De La Recherche Agronomique

Azienda Ospedaliera Papa Giovanni Xxiii

Institut National De La Recherche Scientifique Agronomique (inra)

Jean Kadouche

Kadouche, Jean

Martine Cerutti

Vincent Zoete

Jean-Pierre Mach

Olivier Michielin

Justyna Iwaszkiewicz

Sylvie Choblet

Josee Golay

Gerard Devauchelle

G Erard Devauchelle

Jean-Michel Masson

Thierry Chardes

Laurent Gauthier

Bernard Pau

Claude Granier

Marie-Alix Poul

Hassan Chaabihi

Christian Devaux

Michel Kaczorek

Marie-Paule Lefranc

C Ecile Persillon

Marie-Helene Ogliastro

Laurence Briant-Longuet

Laurence Garnier

Viviane Valverde

Claire Cahoreau

Guy Croizier

Liliane Croizier

Gérard Devauchelle

Cédric Bes

Josée Golay

The invention relates to multispecific antibody constructs comprising Fab fragments having mutations at the interface of the CHI and CL domains, said mutations preventing heavy chain/light chain mispairing.

Multispecific antigens binding fragments and multspecific antibodies

The invention relates to multispecific antibody constructs comprising Fab fragments having mutations at the interface of the CH1 and CL domains, said mutations preventing heavy chain/light chain mispairing.

Multispecific antigens binding fragments and multispecific antibodies

Multispecific antigen binding fragments and multispecific antibodies derived therefrom comprising mutant CH1 and CL-kappa domains

The invention relates to multispecific antibody constructs comprising Fab fragments having mutations at the interface of the CH1 and CL domains, said mutations preventing heavy/light chain mispairing.

Multispecific antigens binding fragments and multispecific antibodies derived therefrom comprising mutant CH1 and CL-&#x3ba; domains

A mutant Fab fragment of the 13B8.2 anti-CD4 antibody that binds a CD4 molecule and includes a mutation of at least one residue in a position situated in the VH variable domain of the heavy chain and/or in a position situated in the V&#x3ba; variable domain of the light chain.

Mutant Fab fragments of the chimeric 13B8.2 anti-CD4 antibody and their applications

Recombinant baculovirus used as an expression vector in the production of immunoglobulins with an insect cell. The invention is characterized by a first expression cassette comprising a first sequence coding for at least one portion of an immunoglobulin heavy chain, wherein the sequence is transcriptionally controlled by a first baculovirus promoter, and a second expression cassette comprising a second sequence coding for at least one portion of an immunoglobulin light chain, said second sequence being transcriptionally controlled by a second baculovirus promoter. The first and second promoters are two different promoters or derivatives of different promoters, the first and second promoters residing at different loci.

Recombinant baculovirus and use thereof in the production of monoclonal antibodies

The use of a prolactin receptor intracytoplasmic domain or a growth hormone receptor intracytoplasmic domain, or of a fragment of either one of said domains, for achieving the secretion of a protein of interest produced in a eukaryotic host cell, is disclosed.

Use of a prolactin receptor or growth hormone receptor intracytoplasmic domain for achieving protein secretion

Method for regulating the expression of a gene in a baculovirus using a

The subject of the invention is the use of a DNA fragment comprising a sequence from the 3' end of the AcNPV baculovirus IE1 gene, optionally combined with a DNA fragment comprising a sequence situated upstream of the promoter of the IE1 gene, for producing vectors permitting the replication and maintenance of genetic information in animal cells. The invention also encompasses the episomal vectors obtained, as well as the transformed cells comprising the vectors.

Nucleotide sequences permitting the replication and maintenance of a

The present invention describes modified baculoviruses possessing unique restriction endonuclease cleavage sites downstream of the promoter of the baculovirus viral inclusion, polyhedron and/or protein P10 genes and a method for obtaining such modified baculoviruses. Such modified baculoviruses are useful for the direct cloning of heterologous DNA into the unique endonuclease restriction sites and the consequent expression of the heterologous DNA inserted therein.

Modified baculovirus, its preparation process and its application as a

Modified baculovirus and baculovirus expression vectors

Plasmid replication origin increasing the copy number of the plasmid

Growing community of inventors

Saint-Christol-lez-Ales, France

Martine Cerutti