Dsm IP Assets B.v.

Martina Beishuizen

Peter Jozef Ida Van De Vondervoort

Noel Nicolaas Maria Elisabeth Van Peij

Noël Nicolaas Maria Elisabeth Van Peij

Cornelis Maria Jacobus Sagt

Robertus Antonius Mijndert Van Der Hoeven

Dick Schipper

Yvonne Johannes Odilia Arendsen

Olaf Leonardus Schouten

Linda De Lange

Serge Petrus Donkers

Marcus Hans

Nöel Nicolaas Maria Elisabeth Van Peij

Van Noël Nicolaas Maria Elisabeth Peij

Van De Peter Jozef Ida Vondervoort

Van Der Robertus Antonius Mijndert Hoeven

De Linda Lange

Noel Nicolaas Van Peij

Peter Jozef Van De Vondervoort

Elisabeth Wilhelmina Maria Van De Vondervoort

The present invention relates to a mutant microbial host cell which is deficient in the production of the AgsE protein or in the production of an homologous thereof if compared with a parent microbial host cell which has not been modified and measured under the same conditions. It has been surprisingly found that when the mutant microbial host cell according to the invention is used in a method to produce a compound of interest, for example an enzyme, an improved yield of said compound is obtained if compared to a method in which a parent host cell which has not been modified is used when measured under the same conditions.

AgsE-deficient strain

The present invention relates to a mutant microbial host cell which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide having &#x3b1;-amylase activity AmyC or an homologous thereof. It has been surprisingly found that when the mutant microbial host cell according to the invention is used in a method to produce a compound of interest, for example an enzyme, an improved purity of the product is obtained.

Amylase-deficient strain

The present invention relates to a method for carrying out recombination at a target locus.

Recombination system

The present invention relates to a method for the production of a compound of interest by microbial fermentation, wherein the microbial host cell used has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The present invention further relates to a microbial host cell that has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The invention further relates to a compound of interest.

Recombinant host cell with deficiency in non-ribosomal peptide synthase production

The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest.

Production of polypeptides by improved secretion

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Echt, Netherlands

Martina Beishuizen