The United States of America As Represented by the Secretary of

Cornell Research Foundation Inc.

Martin Wiedmann

Carl A Batt

Richard N Brandon

Pina M Fratamico

Solomon K Sackitey

Primers specific for enterohemorrhagic Escherichia coli (EHEC) 0157:H7 bacteria have been designed which are useful for detecting the bacteria by polymerase chain reaction methods. The primers were derived from DNA sequences contained within a 60-MDa plasmid which is present in most EHEC. The primers may also be used in combination with primers derived from other sequences of significance, the conserved sequences of Shiga-like toxins I and II and the eaeA gene, in a single simultaneous amplification reaction to specifically identify EHEC serotype 0157.

Assay for enterohemorrhagic Escherichia coli 0157:H7 by the polymerase

The present invention relates to novel compositions comprising Bovine Herpesvirus-1 (BHV-1) specific oligonucleotides which are useful as nested primers to amplify sequences of the BHV-1 gIV gene during enzymatic nucleic acid amplification. The invention also provides a method for the detection of BHV-1 which may be present in a clinical specimen, particularly bovine semen, using the BHV-1 specific nested primers and enzymatic nucleic acid amplification. The present invention also relates to a BHV-1 specific oligonucleotide which can be used as a probe to facilitate detection of amplified products derived from BHV-1 gIV gene sequences.

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Ithaca, NY, United States of America

Martin Wiedmann