Mosaic Biosciences, Inc.

Dexela Limited

Other

Brandeis University

Archemix Corporation

Nec Solution Innovators, Ltd.

Somalogic, Incorporated

Martin Stanton

Alexander Stewart

Peter D Mariner

Alexei V Kazantsev

Edward Bullard

Walter Phillips

Charles Wilson

David Epstein

Thomas Greene McCauley

Dilara Grate

Jeanne Callan

Pieter Wensink

Larry M Gold

Bruce Eaton

Daniel J Schneider

John Louis Diener

Gordon Row

Iwao Waga

Dominic Zichi

Katsunori Horii

Markus Kurz

Thomas Mccauley

Richard Hardwick Moore

Tao Wu

Jeffrey C Kurz

Daniel B Kopans

Dan Nieuwlandt

Stephan Kraemer

Tomoko Fujita

Shashi Gupta

Charles G Ingersoll

Tony Keefe

Errol Desouza

Kristin A Marshall

Alex Stuart

Andrew Dalby

Timothy Lee Moulton

Dilârâ McCauley

Alex Stewart

Nobuko Hamaguchi

Jeffrey Preiss

An adjustable spooling apparatus for storing and unwinding a cable formed into a coil and having a coil diameter, the adjustable spooling apparatus comprising a central hub, a pair of adjustable arms movably engaged with the central hub, and a rotating handle. The adjustable arms each have a coil retention member, and can be selectively extended or retracted to increase or decrease a retention member distance measured between the coil retention members of the two adjustable arms to match the coil diameter. The rotating handle is attached to the central hub, and allows the adjustable spooling apparatus to rotate when the cable is pulled to facilitate unwinding of the coil. The adjustable spooling apparatus further comprises a pair of pivoting arms to prevent the coil from being dislodged, and an engagement point for a power tool to facilitate rapid winding of the cable by rotating the adjustable spooling apparatus.

Adjustable spooling apparatus

The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of: (A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and (B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

Method for producing nucleic acid molecule, biomaterial, and method for producing biomaterial

Provided are methods for linking polypeptides (including peptides and proteins) to other moieties using radical imitated thiol-ene chemistries, for example, modifying a polypeptide by introducing reactive thiol groups and reacting the thiol groups with olefin-containing reagents or alkyne-containing reagents under conditions that support radical thiol-ene or thiol-yne reactions. The reactive thiol groups have greater activity for radical thiol-ene reactions that a cysteine thiol group, including thiol groups that are separated from the peptide backbone by at least two carbon atoms, for example, the thiol group of a homocysteine residue. Also provided are compositions and biomaterials containing the linked polypeptides, for example, peptide and protein conjugates, and thiol-ene based biocompatible hydrogel polymers, and their uses in the medical field.

Degradable thiol-ene polymers and methods of making thereof

The disclosure provides biomaterials including scaffolds that include blood products including blood fractions and products including platelets for administration to subjects in need thereof. More specifically, the scaffolds based on step growth polymers are enriched with blood extracts that contain platelet rich plasma (PRP) and/or extracts of platelets. Compositions comprising a biomaterial or precursor thereof and a blood extract are provided, as are methods of making and using the biopolymers or precursors thereof. Kits and articles of manufacture comprising the biopolymers or precursors thereof are also described.

Biomaterials for delivery of blood extracts and methods of using same

The present disclosure provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a biological macromolecule comprising one or more thiol groups; and (b) a molecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure also provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a molecule comprising one or more thiol groups; and (b) a biological macromolecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure further provides a covalently modified biological macromolecule prepared by any of the disclosed methods. The covalently modified biological macromolecules may be further crosslinked to form a scaffold.

Covalent modification of biological macromolecules

Materials and methods are provided for producing and using aptamers useful as oncology therapeutics capable of binding to PDGF, PDGF isoforms, PDGF receptor, VEGF, and VEGF receptor or any combination thereof with great affinity and specificity. The compositions of the present invention are particularly useful in solid tumor therapy and can be used alone or in combination with known cytotoxic agents for the treatment of solid tumors. Also disclosed are aptamers having one or more CpG motifs embedded therein or appended thereto.

Aptamers comprising CPG motifs

A resizable cord holder for storing and unwinding cord bundles has two elongated C-channel arms connected adjacent to each other to form a supporting base. The arms are connected to each other by a handle, which is installed into corresponding handle holes in the two arms. The total length of the supporting base can be adjusted by reselecting which holes the handle is installed into. The supporting base can rotate relative to the handle for unwinding an installed cord bundle. Cord bundles are held in place by two retainer mounts at opposite ends of the supporting base. A separation member of the retainer mounts traverses through the arms, connecting a locking member and a retainer member on opposite sides of the arms, with the locking members within the C-channels. Coil springs around the separation members apply retaining tension to the locking members against the inner surface of the C-channels.

Resizable cord holder for storing and unwinding cord bundles

The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Multiplexed analyses of test samples

A collapsible cord holder is an apparatus that is specifically designed to prevent entanglements when unwinding an existing cord bundle. The collapsible cord holder accomplishes this through the use of an extendable arm assembly that is pivotally coupled to a handle assembly and secured in both an expanded configuration and a collapsed configuration through a locking mechanism. The arm assembly is able to arcuately expand beyond the diameter of the cord bundle in order to apply tension facilitating the unwinding of the cord bundle. The handle assembly permits an axial rotation of the cord bundle mounted on the collapsible cord holder in order to facilitate unwinding and prevent entanglements. The locking mechanism provides a secure engagement in the expanded configuration allowing the apparatus to transition into the collapsed configuration.

Collapsible cord holder

In one aspect, a method of displaying data is provided. The method comprises obtaining projection data of an object by exposing an object to radiation at a plurality of view angles and detecting at least some of the radiation exiting the object to form the projection data, operating a computer to reconstruct the projection data at a reconstruction resolution to form image data comprising a plurality of voxels representing locations within the object, each of the plurality of voxels being assigned an associated intensity indicative of a density of the subject matter at the respective location, determining a maximum resolution for display, above which variation in intensity between adjacent voxels is not supported by information in the projection data, the maximum resolution being less than the reconstruction resolution, and displaying the image data at or below the maximum resolution.

Methods and apparatus for displaying images

The invention relates to compositions, systems, and methods for simultaneously detecting the presence and quantity of one or more different compounds in a sample using aptamer beacons. Aptamer beacons are oligonucleotides that have a binding region that can bind to a non-nucleotide target molecule, such as a protein, a steroid, or an inorganic molecule. New aptamer beacons having binding regions configured to bind to different target molecules can be used in solution-based and solid, array-based systems. The aptamer beacons can be attached to solid supports, e.g., at different predetermined points in two-dimensional arrays. The invention includes devices, methods, and computer software for carrying out the methods.

Nucleic acid-based detection

Growing community of inventors

Boulder, CO, United States of America

Martin Stanton