Gsf-forschungszentrum Fur Umwelt Und Gesundheit, Gmbh

Gsf-forschungszentrum Fuer Umwelt Und Gesundheit Gmbh

Martin Staege

Georg W Bornkamm

Bettina Kempkes

Wolfgang Hammerschmidt

Axel Polack

Ursula Zimber-Strobl

T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desired antigen; and (g) optionally purifying and isolating the stimulated T cells.

Method for the stimulation of T cells having a desired antigen specificity

The invention is directed to a method for the preparation of a conditionally immortalized immortalization-helper cell (fuseme), the fusemes generated by said method, hybridoma cells prepared using said fusemes as well as a method for immortalization of mammalian cells using said fuseme cells. Further, the invention relates to the generation of T cells directed agaist tumor cells using a fuseme cell.

Method for the immortalization of cells using conditionally transformed helper cells

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Munich, Germany

Martin Staege