Pall Corporation

Yale University

Martin Smith

Xiaosong Wu

Christopher K Breuer

Geoffrey Patrick Larue

Keru O Shafi

Edward L Snyder

Asymmetric microporous membranes with localized asymmetries, devices including the membranes, and methods of making and using the membranes, are disclosed. The asymmetric microporous membranes have a first surface and a second surface and an interior bulk defined by the first and second surfaces, wherein the first surface has at least a first region and a second region, the first region having a first porosity comprising a first mean pore size, and the second region having a second porosity comprising a second mean pore size, wherein the ratio of first mean pore size to the second mean pore size is at least about 5:1.

Membrane with localized asymmetries

Systems are provided for convenient and sterile isolation, collection, and seeding of cells onto a scaffold or tissue graft. The systems may be closed. Methods for use of the disclosed systems for isolation, collection and seeding of cells and generation of tissue engineered vascular grafts are also provided. The systems may be supplied in kits for efficient and expeditious use.

System for seeding cells onto three dimensional scaffolds

Growing community of inventors

Northport, NY, United States of America

Martin Smith