Kapa Biosystems, Inc.

Roche Sequencing Solutions, Inc.

Martin Ranik

Paul McEwan

Eric Van Der Walt

Ashley Hayes

Severine Margeridon

Jenny A Johnson

Richard Dannebaum

Daleen Badenhorst

Aruna Arcot

Persis Wadia

Jennifer Hsieh

William Bourn

Rui Chen

Alexandra Hui Wang

Ross Wadsworth

Monica Herrera

Marliz Strydom

Toummy Guettouche

Michael S Berry

Etienne Slabbert

Gerrida Uys

Abre De Beer

Paul Mcewan

Suzanne Huddy

Du Toit Schabort

Michael Stuart Berry

Raeeza Allie

The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of single stranded circular target nucleic acids.

Generation of single-stranded circular DNA templates for single molecule sequencing

The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of double stranded target nucleic acids containing a linear partially single-stranded adaptor.

Generation of double-stranded DNA templates for single molecule sequencing

Disclosed is a method of fragmenting DNA comprising contacting a sample of target DNA with (a) a composition comprising an active transpososome, and (b) a composition comprising an inactive transpososome, under conditions suitable for transpososome activity, wherein a ratio of an amount of the inactive transpososome in the composition of (b) to an amount of the active transpososome in the composition of (a) determines the mean fragment size and a level of insertion bias.

Transposase competitor control system

The invention is a novel method of separately sequencing each strand of a nucleic acid involving the use of an adaptor comprising a strand cleavage site or a strand synthesis termination site. The adaptor may also be self-priming at the strand cleavage site.

The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5' to 3&#x2032;, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.

System and method for nucleic acid library preparation via template switching mechanism

The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.

Hybridization-extension-ligation strategy for generating circular single-stranded DNA libraries

The present invention provides synthetic nucleic acid molecule tags that can be added into samples for identification and tracking. Among other things, the present invention provides synthetic, high-molecular weight concatemers, which can be combined with samples to yield hundreds of millions of unique identifiers. Examples applications for which the synthetic nucleic acid molecule tags can be used, include industrial, research and clinical applications.

High-molecular weight DNA sample tracking tags for next generation sequencing

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Cape Town, South Africa

Martin Ranik