Mount Sinai Hospital Corporation

The Hospital for Sick Children

Other

Martin Post

Mount Sinal Hospital

The Hosptial for Sick Children

Isabella Caniggia

Stephen Lye

Michael Litvack

Sheena Bouch

A method of differentiating pluripotent stem cells into hemangioblasts comprising incubating the pluripotent stem cells in a first serum-free differentiation medium comprising bone morphogenetic protein 4 (BMP4), vascular endothelial growth factor (VEGF) and stem cell factor (SCF) to induce differentiation of the pluripotent stem cells into hemangioblasts or hemangioblast-containing embryoid bodies is provided. The hemangioblasts or embryoid bodies may be cultured in a second differentiation medium comprising at least granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (M-CSF) and interleukin-3 (IL-3) for a period of time sufficient to generate alveolar-like macrophages.

Method of generating hemangioblasts

Alveolar-like macrophages and a method for generating alveolar-like macrophages from hemangioblasts is provided. The method comprises the steps of: i) culturing the hemangioblasts in a hematopoietic-inducing medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF) and interleukin-3 (IL-3) for a sufficient period of time to generate macrophages, and ii) culturing the macrophages in an alveolar macrophage-inducing medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), and optionally macrophage colony stimulating factor (M-CSF), under suitable conditions and for a sufficient period of time to yield alveolar-like macrophages.

Mammalian alveolar macrophages derived from pluripotent cells

The invention relates to reagents and methods for detecting, diagnosing and screening for conditions associated with hydroxylated hypoxia inducible factor 1-&#x3b1; (HIF-1&#x3b1;). The invention also relates to novel monoclonal antibodies specific for hydroxylated HIF-1&#x3b1;, or binding fragments thereof, and related nucleic acids, vectors, cells and compositions, as well as methods of using the antibodies in methods of the invention.

Antibodies against human HIF-1&#x3b1;

Methods are provided for the diagnosis and treatment of patients with increased risk of preeclampsia. The methods involve measuring levels of TGF-&#x3b2;, receptors of cytokines of the TG&#x3b2; family, or HIF-1&#x3b1;.

Method for diagnosing an increased risk of preeclampsia by measuring the level of HIF-&#x3b1;

Methods for early diagnosing of an increased risk of preeclampsia

Methods are provided for the diagnosis and treatment of patients with increased risk of preeclampsia. The methods involve measuring levels of TGF-beta 3, receptors of cytokines of the TG beta family, or HIF-1 alpha.

Method for diagnosing increased risk of preeclampsia

Methods to diagnose a required regulation of trophoblast invasion

Methods are provided for the diagnosis and treatment of patients with increased risk of preeclampsia. The methods involve measuring levels of TGF-&bgr; , receptors of cytokines of the TG&bgr; family, or HIF-1&agr;.

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Toronto, Canada

Martin Post