Singular Genomics Systems, Inc.

Illumina Corporation

Martin Maria Fabani

Eli N Glezer

Daan Witters

Sabrina Shore

John A Moon

Maria Candelaria Rogert Bacigalupo

Ryan Shultzaberger

Gudrun Stengel

Bharat Sridhar

Christopher Jen-Yue Wei

Timothy Looney

Yeoan Youn

Andrew Pawlowski

Michael Lawson

Disclosed herein, inter alia, are methods for sequencing a target sequence in a cell by forming a circular oligonucleotide that incorporates a complement of the target sequence.

Sequencing a target sequence in a cell

Provided herein are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including interposing oligonucleotide barcodes, and kits useful in obtaining long-range sequence data.

Polynucleotide barcodes for long read sequencing

Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.

Linked paired strand sequencing

A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.

Sequencing by orthogonal synthesis

Growing community of inventors

Encinitas, CA, United States of America

Martin Maria Fabani