The General Hospital Corporation

Rutgers, the State University of New Jersey

Massachusetts Institute of Technology

Other

L'oreal

Northeastern University

William Marsh Rice University

Vasculogic, LLC

Hurel Corporation

Loreal Sa

Martin L Yarmush

Rene S Schloss

Mehmet Toner

Tim Maguire

Jeffrey R Morgan

Maria-Louisa Izamis

Osman Berk Usta

Basak Elif Uygun

Sangeeta N Bhatia

William G Austen

Alvin Chen

Nir I Nativ

Ronald G Tompkins

Mustafa Korkut Uygun

Alexander Golberg

Gabriel A Yarmush

Timothy Antonie Berendsen

Yaakov Nahmias

Francois Berthiaume

William Lee

Ulysses J Balis

Boris Rubinsky

Michael Belkin

Mark A Latina

Erkin Seker

Robert Marius Bieganski

Biju Parekkadan

Tali Konry

Eric Novik

Robert Freedman

James Dunn

Sinem Perk

Joseph M Le Doux

Arno W Tilles

Alan J Grodzinsky

Maury D Cosman

William Gerald Austen, Jr

Tania Konry

Paul A DiMilla

Kevin R King

Piyush Koria

Robert L Sheridan

Saiqa I Khan

William C Olson

Paul E Grimshaw

Rohit Jindal

Max Balter

Anil Shrirao

Ileana Marrero-Berrios

Haishui Huang

Korkut Uygun

G Felix Broelsch

Jean-Pierre Dolle

Joshua Leipheimer

Kevin Nikitczuk

Stanley Dunn

Saiqa L Khan

Aurelia Del Bufalo

Alejandro Soto-Gutierrez

Silvia Teissier

Jean-Roch Meunier

Bhaskar Mitra

Eric Yang

Dhruv Sarin

Herman Tolboom

Prakash Rai

Mehdi Ghodbane

Yevgeny Berdichevsky

Eric J Wallenstein

Hiroshi Yagi

Deepak Nagrath

Carley Shulman

Marco Avila-Elchiver

Jack Milwid

Cheul Cho

Alexander Goldberg

Amit Parekh

Eric Pludwinski

Suraj J Patel

John M Milwid

This disclosure relates to methods of super-cooling of aqueous samples with or without biological samples. The methods involve, e.g., providing a container comprising the aqueous sample; applying an immiscible liquid phase of sufficient thickness to separate the aqueous sample from air; and cooling the aqueous sample to a temperature that is below 0° C.

Methods of supercooling aqueous samples

A device and method for directing a cannula toward a target location under a patient's skin. The device is handheld and the device operations may be automated. The device includes an imaging probe for imaging the target location, a positioning unit for manipulating a cannula towards the target location, and a processor. The processor receives imaging data from the imaging probe, cannula pose data from at least one cannula position sensor, and device pose data from at least one device position sensor. The target location is identified from the imaging data, and a trajectory for manipulating the cannula towards the target location is determined based on the imaging data, the cannula pose data, and the device pose data. The processor may determine that the trajectory becomes misaligned with the target position, and may update to a corrected trajectory based on the imaging data, cannula pose data, and device pose data.

Ultrasound-guided alignment and insertion of percutaneous cannulating instruments

The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Methods and compositions for preserving tissues and organs

Described herein is a cell culture device and methods of use in three-dimensional cell co-cultures and for use in studying paracrine signaling in vitro.

Multilayer stackable tissue culture platform for 3D co-culture

A device and method for directing a cannula toward a target location under a patient's skin. The device is handheld and the device operations may be automated. The device includes an imaging probe for imaging the target location, a positioning unit for manipulating a cannula towards the target location, and a processor. The processor receives imaging data from the imaging probe, cannula pose data from at least one cannula position sensor, and device pose data from at least one device position sensor. The target location is identified from the imaging data, and a trajectory for manipulating the cannula towards the target location is determined based on the imaging data, the cannula pose data, and the device pose data. The processor may determine that the trajectory becomes misaligned with the target position, and may update to a corrected trajectory based on the imaging data, cannula pose data, and the device pose data.

Microfluidic devices for use with reagents bound to microspheres for determination of the concentration of an analyte in a liquid sample are provided. The devices include two sequential mixing channels that promote rapid binding of microsphere-bound reagents with reagents in solution and a means for detecting labeled microsphere-bound reaction products. Also provided are methods for using the devices with microsphere-bound reagents to determine the concentration of an analyte in a liquid sample and to measure the binding affinity of antibody for an antigen.

Microfluidic system and method for real-time measurement of antibody-antigen binding and analyte detection

A system and method for controlling microbial growth on and in medical devices and implants, especially biofilm infections, involves using pulsed electric fields (PEF). To eradicate at least a portion of a biofilm on a medical implant, for example, 1500 volts can be applied through an electrode system, with pulse duration of 50 &#x3bc;s and pulse delivery frequency of 2 Hz. In the clinical setting, systemic microbial therapy can be combined with PEF to achieve a synergistic effect leading to improved eradication of infections.

System and method for electrical control of bacteria

One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same

This application discloses alginate microencapsulation-mediated differentiation of embryonic stem cells and use of the stem cell differentiation method for the development of effective treatment of various diseases and disorders. The microencapsulation of embryonic stem (ES) cells results in decreased cell aggregation and enhanced neural lineage differentiation through incorporating the soluble inducer retinoic acid (RA) into the permeable microcapsule system. This differentiation process can be augmented by differentiation pathway regulators such as PPAR agonists.

Lineage differentiation of encapsulated embryonic stem cells

The present invention relates to a system and methods for identifying a compound for de-fatting and functional recovery of macrosteatotic hepatocytes.

In vitro model of macrosteatotic (fatty) liver

Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.

Compositions, methods, and devices for treating disease

This invention discloses devices and methods for high throughput skin sensitization detection. The devices comprise a microfabricated chamber comprising a region having one or more input channels and an outlet, and a face suitable for mounting a skin tissue and in fluidic communication with the region. The devices can be used in the methods for determining a prognosis of sensitization in an animal subject and identifying compounds that do not cause sensitization and thus are suitable for preparing cosmetic compositions.

High throughput sensitization detection devices and methods

Systems and methods for controlling a tissue of a subject using applied pulsed electric fields. The system for controlling a therapy provided to a tissue of a subject using applied pulsed electric fields. The system includes an electrode assembly configured to engage a skin tissue of a subject to deliver a series of electric field pulses to the skin tissue and a user input configured to receive an operational instruction for the series of electric field pulses. The operational instruction defines at least one of a pulse duration, a pulse frequency, a pulse number, and a pulse amplitude. The system also includes at least one processor configured to access the operational instruction received by the user input and, using the operational instruction, create an electric field profile to be generated by the electrode assembly about the skin tissue of the subject to control a fibroblast characteristic while preserving a vascular perfusion in at least a portion of the skin tissue. The processor is also caused to control the electrode assembly using the electric field profile to deliver the series of electric field pulses to control the fibroblast characteristic.

Systems and methods for delivering pulsed electric fields to skin tissue

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Sharon, MA, United States of America

Martin L Yarmush