Luminex Corporation

Martin Kochanczyk

Doug Whitman

Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5' of the target-specific primer region; and a second stem forming region 3&#x2032; of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

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Austin, TX, United States of America

Martin Kochanczyk