Burroughs Wellcome Co.

Martin J Page

Ian George Charles

Brian C Rodgers

Timothy C Peakman

The invention relates to a CHO cell-line capable of producing antibody, the cell-line having been co-transfected with a vector capable of expressing the light chain of the antibody and a vector capable of expressing the heavy chain of the antibody wherein the vectors contain independently selectable markers; also included is a CHO cell-line capable of producing a human antibody or an altered antibody, the cell-line having been transfected with a vector capable of expressing the light chain of the antibody and the heavy chain of the antibody; process for the production of antibody using a CHO cell-line and antibody having CHO glycosylation.

Method for treating a mammal by administering a CHO-glycosylated antibody

Method for treating a mammal suffering from a T-cell medicated disorder

Method for treating a mammal suffering from cancer with a

A process for the preparation of a protein, which process comprises culturing under such conditions that the protein is obtained cells infected with a recombinant baculovirus having a promoter, capable of directing the expression of a gene in the cells, operably linked to a heterologous gene which encodes the protein and which is preceded by a leader sequence comprising: T A A T C A T C C A C A G G A G A C T T T C T G [SEQ. ID NO: 1].

Procaryotic leader sequence in recombinant baculovirus expression system

A baculovirus transfer vector incorporates a restriction site into which a foreign gene may be cloned a short distance downstream of the N-terminus of the polyhedrin gene body and the natural ATG translation start codon for the polyhedrin gene is not provided such that the N-terminal polyhedrin coding sequence prior to the restriction site is retained but not capable of being translated.

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Beckenham, United Kingdom

Martin J Page