Dsm IP Assets B.v.

Evonik Operations Gmbh

Martin Heining

Michael B Johnson

Vinod Tarwade

Neil Francis Leininger

Ginger Marie Shank

Kirt Lyvell Matthews, Sr

Holger Pfeifer

Jochen Lebert

Annika Heining

Christian Rabe

Nasrin Tabayehnejad

Xiao Daniel Dong

David Allen Tinsley

Shannon Elizabeth Ethier Resop

Michael Diehl

Mark Edward Nejako, Ii

Viktor Stefan Wallimann

Annika Hartmann

Negash Adugna

Kirt Lyvell Matthews

Michael Benjamin Johnson

Shannon Elizabeth Resop

Mark Edward Nejako

Sr Kirt Lyvell Matthews

Mark Edward Nejako Ii

Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Method of reducing emulsion by broth washing

The current invention relates to a method of separating lipids from a lipids containing biomass by demulsification under mild conditions, to an oil and to a delipidated biomass as obtained by such a method.

Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion

The present invention relates to an oil comprising at least one polyunsaturated fatty acid having at least 20 carbon atoms (LC-PUFA). It is found that LC-PUFA-containing oil are susceptible to gelling by formation of microscopic crystals during storage ultimately resulting in unfavorable quality and handling properties. This problem has been particularly observed with a microbial oil comprising at least about 25% by weight LC-PUFA and a moisture content of 0.2 to 5% by weight. Surprisingly, it has been found that a LC-PUFA-containing oil as described above is effectively stable and does not show gelling properties under conventional storage conditions, if the oil composition as such contains less than about 8% preferably less than about 5% by weight of free fatty acid in the residual moisture of the oil. Therefore, the present invention is directed to an oil comprising at least about 25% by weight LC-PUFA and a moisture content comprising less than about 8% preferably less than about 5% by weight of free fatty acid.

Oil comprising at least one polyunsaturated fatty acid having at least 20 carbon atoms (LC-PUFA)

The current invention relates to a method of isolating polyunsaturated fatty acids containing lipids from a lipids containing biomass. The method involves providing a suspension of cells with a PUFAs-containing lipid; optionally lysing the cells; concentrating the suspension; adding base equivalents to the suspension based on the total dry matter present; feeding a continuous reactor with the suspension; and separating the lipids-containing light phase from the water and cell debris-containing heavy phase.

Method of isolating lipids from a lipids containing biomass

The current invention relates to a method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica and to an oil and to a delipidated biomass as obtained by such a method.

Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

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Karlstein am Main, Germany

Martin Heining